-
Article
Open AccessExtracellular vesicle approach to major psychiatric disorders
Over the last few years, extracellular vesicles (EVs) have received increasing attention as potential non-invasive diagnostic and therapeutic biomarkers for various diseases. The interest in EVs is related to ...
-
Article
Open AccessComparative serum proteomic analysis of a selected protein panel in individuals with schizophrenia and bipolar disorder and the impact of genetic risk burden on serum proteomic profiles
The diagnostic criteria for schizophrenia (SCZ) and bipolar disorder (BD) are based on clinical assessments of symptoms. In this pilot study, we applied high-throughput antibody-based protein profiling to seru...
-
Article
Open AccessSpironolactone alleviates schizophrenia-related reversal learning in Tcf4 transgenic mice subjected to social defeat
Cognitive deficits are a hallmark of schizophrenia, for which no convincing pharmacological treatment option is currently available. Here, we tested spironolactone as a repurposed compound in Tcf4 transgenic m...
-
Article
Open AccessPolygenic burden associated to oligodendrocyte precursor cells and radial glia influences the hippocampal volume changes induced by aerobic exercise in schizophrenia patients
Hippocampal volume decrease is a structural hallmark of schizophrenia (SCZ), and convergent evidence from postmortem and imaging studies suggests that it may be explained by changes in the cytoarchitecture of ...
-
Article
Open AccessCorrection to: Monitoring activities of receptor tyrosine kinases using a universal adapter in genetically encoded split TEV assays
The article Monitoring activities of receptor tyrosine kinases using a universal adapter in genetically encoded split TEV assays, written by Jan P. Wintgens, Sven P. Wichert, Luksa Popovic, Moritz J. Rossner a...
-
Article
Open AccessSingle cell RNA-sequencing identified Dec2 as a suppressive factor for spermatogonial differentiation by inhibiting Sohlh1 expression
Gonocyte-to-spermatogonia transition is a critical fate determination process to initiate sperm production throughout the lifecycle. However, the molecular dynamics of this process has not been fully elucidate...
-
Article
Open AccessMonitoring activities of receptor tyrosine kinases using a universal adapter in genetically encoded split TEV assays
Receptor tyrosine kinases (RTKs) play key roles in various aspects of cell biology, including cell-to-cell communication, proliferation and differentiation, survival, and tissue...
-
Article
Open AccessPathway sensor-based functional genomics screening identifies modulators of neuronal activity
Neuronal signal transduction shapes brain function and malfunction may cause mental disorders. Despite the existence of functional genomics screens for proliferation and toxicity, neuronal signalling has been ...
-
Article
Open AccessStudying and modulating schizophrenia-associated dysfunctions of oligodendrocytes with patient-specific cell systems
Postmortem studies in patients with schizophrenia (SCZ) have revealed deficits in myelination, abnormalities in myelin gene expression and altered numbers of oligodendrocytes in the brain. However, gaining mec...
-
Article
Open AccessMultiplexed profiling of GPCR activities by combining split TEV assays and EXT-based barcoded readouts
G protein-coupled receptors (GPCRs) are the largest class of cell surface receptors and are implicated in the physiological regulation of many biological processes. The high diversity of GPCRs and their physio...
-
Article
Essential role for the transcription factor Bhlhe41 in regulating the development, self-renewal and BCR repertoire of B-1a cells
B-1a B cells are a distinct subset of mature B cells that provide innate-like protection against pathogens. Busslinger and colleagues identify the transcription factor Bhlhe41 as being essential for B-1a devel...
-
Protocol
Characterizing Dynamic Protein–Protein Interactions Using the Genetically Encoded Split Biosensor Assay Technique Split TEV
Dynamic protein–protein interactions (PPIs) are fundamental building blocks of cellular signaling and monitoring their regulation promotes the understanding of signaling in health and disease. Genetically enco...
-
Article
A neuronal PI(3,4,5)P3-dependent program of oligodendrocyte precursor recruitment and myelination
In the CNS, the primary signal initiating myelination and its cellular origin remain unclear. Goebbels et al. find that deleting PTEN from cerebellar granule cells drives radial axon growth, oligodendrocyte proge...
-
Article
Age-related myelin degradation burdens the clearance function of microglia during aging
Safaiyan et al. demonstrate that myelin fragments progressively pinch off from aged myelin sheaths and are taken up and cleared by microglia. Age-associated myelin breakdown is substantial and saturates the degra...
-
Article
Cell type– and brain region–resolved mouse brain proteome
The authors performed a comprehensive proteome analysis of the adult mouse brain, its major regions and CNS cell types at a depth of >13,000 proteins. This new resource represents the largest collection of cel...
-
Article
Dissecting the regional diversity of glial cells by applying -omic technologies
Neuronal as well as glial cells contribute to higher order brain functions. Many observations show that neurons and glial cells are not only physically highly intermingled but are physiologically tightly conne...
-
Article
Data calibration and reduction allows to visualize behavioural profiles of psychosocial influences in mice towards clinical domains
Psychosocial stress—particularly in combination with genetic vulnerability—is a critical environmental risk factor for psychiatric diseases in humans. Isolation rearing (IR) and social defeat (SD) paradigms mo...
-
Protocol
Monitoring G Protein-Coupled Receptor Activation Using the Protein Fragment Complementation Technique Split TEV
G protein-coupled receptors (GPCRs) modulate cellular signaling, often in a ligand-specific manner. Cellular effects regulated include differentiation, proliferation, hormonal regulation, and neuronal activity...
-
Article
Open AccessThe multispecific thyroid hormone transporter OATP1C1 mediates cell-specific sulforhodamine 101-labeling of hippocampal astrocytes
Sulforhodamine 101 (SR101) is widely used for astrocyte identification, though the labeling mechanism remains unknown and the efficacy of labeling in different brain regions is heterogeneous. By combining regi...
-
Article
Transcription factor 4 (TCF4) and schizophrenia: integrating the animal and the human perspective
Schizophrenia is a genetically complex disease considered to have a neurodevelopmental pathogenesis and defined by a broad spectrum of positive and negative symptoms as well as cognitive deficits. Recently, la...
