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Article
Open AccessTargeted suppression of mTORC2 reduces seizures across models of epilepsy
Epilepsy is a neurological disorder that poses a major threat to public health. Hyperactivation of mTOR complex 1 (mTORC1) is believed to lead to abnormal network rhythmicity associated with epilepsy, and its ...
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Article
Open AccessA cross-species spatiotemporal proteomic analysis identifies UBE3A-dependent signaling pathways and targets
Angelman syndrome (AS) is a severe neurodevelopmental disorder caused by the loss of neuronal E3 ligase UBE3A. Restoring UBE3A levels is a potential disease-modifying therapy for AS and has recently entered cl...
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Article
Multiplexed proteome analysis with neutron-encoded stable isotope labeling in cells and mice
This NeuCode SILAC/SILAM protocol uses neutron-coded lysine derivatives to improve stable isotope labeling of amino acids in cells or mice, enabling multiplexed comparison of proteomes from different samples b...
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Protocol
Perseus: A Bioinformatics Platform for Integrative Analysis of Proteomics Data in Cancer Research
Mass spectrometry-based proteomics is a continuously growing field marked by technological and methodological improvements. Cancer proteomics is aimed at pursuing goals such as accurate diagnosis, patient stra...
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Article
The MaxQuant computational platform for mass spectrometry-based shotgun proteomics
MaxQuant is a platform for mass spectrometry-based proteomics data analysis. It includes a peptide database search engine, called Andromeda, and expanding capability to handle data from most quantitative prote...
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Article
The Perseus computational platform for comprehensive analysis of (prote)omics data
Perseus is a comprehensive, user-friendly software platform for the biological analysis of quantitative proteomics data. It is intended to help biologists with little bioinformatics training to interpret prote...
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Article
Open AccessProteomic maps of breast cancer subtypes
Systems-wide profiling of breast cancer has almost always entailed RNA and DNA analysis by microarray and sequencing techniques. Marked developments in proteomic technologies now enable very deep profiling of ...
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Article
Cell type– and brain region–resolved mouse brain proteome
The authors performed a comprehensive proteome analysis of the adult mouse brain, its major regions and CNS cell types at a depth of >13,000 proteins. This new resource represents the largest collection of cel...
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Protocol
MaxQuant for In-Depth Analysis of Large SILAC Datasets
Proteomics experiments can generate very large volumes of data, in particular in situations where within one experimental design many samples are compared to each other, possibly in combination with pre-fracti...
