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Article
Favorable NK cell activity after haploidentical hematopoietic stem cell transplantation in stage IV relapsed Ewing’s sarcoma patients
Natural killer (NK) cell activity has been shown to have potential activity against Ewing’s sarcoma (EWS) especially in tumors with low HLA I expression and high NKG2D expression. Two patients with metastatic ...
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Article
Improved immune recovery after transplantation of TCRαβ/CD19-depleted allografts from haploidentical donors in pediatric patients
Immune recovery was retrospectively analyzed in a cohort of 41 patients with acute leukemia, myelodysplastic syndrome and nonmalignant diseases, who received αβ T- and B-cell-depleted allografts from haploiden...
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Article
IL-15-stimulated CD3/CD19-depleted stem-cell boosts in relapsed pediatric patients after haploidentical SCT
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Article
Immune reconstitution after haploidentical hematopoietic cell transplantation: impact of reduced intensity conditioning and CD3/CD19 depleted grafts
Haploidentical hematopoietic cell transplantation (HHCT) using CD34 selected grafts is complicated by slow engraftment and immune reconstitution. Engraftment and immune reconstitution might be improved using C...
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Article
Cytolytic activity of NK cell clones against acute childhood precursor-B-cell leukaemia is influenced by HLA class I expression on blasts and the differential KIR phenotype of NK clones
Relapse after allo-SCT in patients with acute leukaemia remains a major problem. A beneficial impact of alloreactive natural killer (NK) cells has been reported for myeloid malignancies, but has been questiona...
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Article
Flow cytometry with anti HLA-antibodies: a simple but highly sensitive method for monitoring chimerism and minimal residual disease after HLA-mismatched stem cell transplantation
Transplantation of HLA-mismatched stem cells may allow determination of chimerism status of single cells by differential expression of HLA molecules. Monoclonal antibodies against HLA antigens can be used to d...
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Article
Bearbeitung und Transplantation hämatopoetischer Stammzellen
Neue Methoden zur Anreicherung und Depletion definierter Zellpopulationen haben es möglich gemacht, hämatopoetische Stammzellen in hoher Reinheit von gesunden Spendern zu gewinnen.
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Article
Correction of persistent thrombocytopenia by a boost of CD133+ selected stem cells in a patient transplanted for Wiskott-Aldrich syndrome 10 years ago
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Article
Transplantation of CD34+ enriched allografts in children with nonmalignant diseases: does graft manipulation necessarily result in high incidence of graft failure?
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Article
Transplantation of highly purified peripheral-blood CD34+ progenitor cells from related and unrelated donors in children with nonmalignant diseases
Transplantation of allogeneic stem cells is currently the only curative treatment for some nonmalignant pediatric diseases. We investigated whether transplantation of purified CD34+ stem cells prevents acute and ...
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Article
Successful unrelated allogeneic stem cell transplantation after treatment of lymphoid blast crisis CML with imatinib and imatinib-containing conditioning regimen in a 16-year-old male
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Article
A prospective comparison of immune reconstitution in pediatric recipients of positively selected CD34+ peripheral blood stem cells from unrelated donors vs recipients of unmanipulated bone marrow from related donors
Positively selected CD34+ hematopoietic stem cells from unrelated donors (UD-HSCT) have been successfully transplanted, but little is known about immune reconstitution in this setting. Here we report a prospectiv...
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Chapter and Conference Paper
Immune Reconstitution after Haploidentical Stem Cell Transplantation
With increasing efficacy and safety of the procedure in recent years, the transplantation of hematopoietic stem cells has become an important therapeutic modality for patients with a variety of malignant and n...
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Article
Isolation and transplantation of highly purified autologous peripheral CD34+ progenitor cells: purging efficacy, hematopoietic reconstitution and long-term outcome in children with high-risk neuroblastoma
We have investigated the purging efficacy of positive selection of autologous mobilized CD34+ peripheral stem cells in 22 children with high-risk neuroblastoma. CD34+ cell selection was performed using the method...
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Article
Clinical scale isolation of T cell-depleted CD56+ donor lymphocytes in children
We present a clinical scale method for immunomagnetic separation of CD56+ donor natural killer cells for adoptive immunotherapy of pediatric leukemias after allogeneic transplantation. This time-saving and partia...
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Article
Megadose transplantation of purified peripheral blood CD34+progenitor cells from HLA-mismatched parental donors in children
We performed HLA-mismatched stem cell transplantation with megadoses of purified positively selected mobilized peripheral blood CD34+ progenitor cells (PBPC) from related adult donors in 39 children lacking an ot...
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Article
Unrelated partially matched peripheral blood stem cell transplantation with highly purified CD34+ cells in a child with Wiskott–Aldrich syndrome
Stem cell transplantation is the only curative approach to the treatment of Wiskott–Aldrich syndrome. However, using grafts from partially matched unrelated donors is associated with increased risk of graft re...
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Article
Unrelated peripheral blood stem cell transplantation with ‘megadoses’ of purified CD34+ cells in three children with refractory severe aplastic anemia
Three children with refractory severe aplastic anemia were transfused with high numbers of unrelated matched (n = 2) or C-locus haploidentical mismatched (n = 1) CD34-selected peripheral blood stem cells in the a...
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Chapter
Positive Selection of Hematopoietic Progenitor Cells for Autologous and Allogeneic Transplantation in Pediatric Patients with Solid Tumors and Leukemia
CD34+ progenitor cells were collected by peripheral blood stem cell pheresis (PBSC) from 7 patients with solid tumors (6 neuroblastomas and 1 rhabdomyosarcoma) and from one healthy, allogeneic donor after mobiliz...
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Article
Clinical scale isolation of highly purified peripheral CD34+progenitors for autologous and allogeneic transplantation in children
We present our experience with three clinical scale isolation methods for positive selection of CD34+ progenitors from peripheral blood for autologous and allogeneic transplantation in children. A combination of ...
