22 Result(s)

Article

Pre-emptive immunotherapy with purified natural killer cells after haploidentical SCT: a prospective phase II study in two centers

Adoptive immunotherapy with allogeneic purified natural killer (NK) cell products might exert graft-versus-tumor alloreactivity with little risk of GVHD. In a prospective phase II study in two centers, we admi...

M Stern, J R Passweg, S Meyer-Monard, R Esser, T Tonn… in Bone Marrow Transplantation (2013)

Article

Multivariate analyses of immune reconstitution in children after allo-SCT: risk-estimation based on age-matched leukocyte sub-populations

The speed of immune recovery after allo-SCT is of central importance to overcome infectious complications and relapse. To evaluate the immune reconstitution of pediatric patients concerning overall survival, w...

M Koenig, S Huenecke, E Salzmann-Manrique, R Esser… in Bone Marrow Transplantation (2010)

Article

Natürliche Killerzellen in der Leukämie- und Tumortherapie

Humane natürliche Killerzellen (NK-Zellen) machen 10–15% der Lymphozyten aus und sind als Zellen des angeborenen Immunsystems für die Abwehr von Infektionen und malignen Erkrankungen essenziell. In vitro und i...

Prof. Dr. P. Lang, S. Huenecke, M. Pfeiffer… in Monatsschrift Kinderheilkunde (2010)

Article

Enrichment of cell subpopulations applying automated MACS technique: purity, recovery and applicability for PCR-based chimerism analysis

Enrichment of cell subpopulations is a prerequisite for lineage-specific chimerism analysis (LCA), a frequent approach in follow-up after allo-SCT. An efficient enrichment technique is Magnetic Cell Sorting (M...

A Willasch, S Eing, G Weber, S Kuçi, G Schneider… in Bone Marrow Transplantation (2010)

Article

New insights to the MLL recombinome of acute leukemias

Chromosomal rearrangements of the human MLL gene are associated with high-risk pediatric, adult and therapy-associated acute leukemias. These patients need to be identified, treated appropriately and minimal resi...

C Meyer, E Kowarz, J Hofmann, A Renneville, J Zuna, J Trka, R Ben Abdelali… in Leukemia (2009)

Article

Clinical-scale generation of human anti-Aspergillus T cells for adoptive immunotherapy

Invasive aspergillosis is a major cause of morbidity and mortality in patients undergoing allogeneic hematopoietic SCT. There is a growing body of evidence that T cells are important in the host defense against A...

L Tramsen, U Koehl, T Tonn, J-P Latgé, F R Schuster… in Bone Marrow Transplantation (2009)

Article

ISHAGE-based single-platform flowcytometric analysis for measurement of absolute viable T cells in fresh or cryopreserved products: CD34/CD133 selected or CD3/CD19 depleted stem cells, DLI and purified CD56⁺CD3⁻ NK cells

Considering the growing use of immunotherapeutic strategies in paediatric stem cell transplantation associated with risk of graft-versus-host disease, an accurate method for the enumeration of residual T cells...

U. Koehl, K. Bochennek, R. Esser, A. Brinkmann… in International Journal of Hematology (2008)

Article

Effective treatment of leukemic cell lines with wt1 siRNA

The expression of wt1 and bcl-2 is considered to have a proliferating and survival supporting effect in leukemia blast cells. Here we describe the use of siRNA against wt1 and bcl-2 in leukemic cell lines for suc...

W Glienke, L Maute, U Koehl, R Esser, E Milz, L Bergmann in Leukemia (2007)

Article

Pharmacology of intracellular cytosine-arabinoside-5′-triphosphate in malignant cells of pediatric patients with initial or relapsed leukemia and in normal lymphocytes

The prodrug cytosinearabinoside (ara-C) is widely used in the treatment of acute leukemias. The active drug is the intracellular metabolite cytosine-arabinoside-5′-triphosphate (ara-CTP). The purpose of the pr...

U. Koehl, G. Hollatz, E. Rohrbach, K. Visschedyk… in Cancer Chemotherapy and Pharmacology (2007)

Article

Immune recovery in children undergoing allogeneic stem cell transplantation: absolute CD8⁺CD3⁺ count reconstitution is associated with survival

To evaluate the correlation between kinetics of immune reconstitution and survival, we prospectively evaluated lymphocyte subsets in 32 paediatric patients undergoing allogeneic stem cell transplantation (SCT)...

U Koehl, K Bochennek, S Y Zimmermann, T Lehrnbecher… in Bone Marrow Transplantation (2007)

Article

The MLL recombinome of acute leukemias

Chromosomal rearrangements of the human MLL gene are a hallmark for aggressive (high-risk) pediatric, adult and therapy-associated acute leukemias. These patients need to be identified in order to subject these p...

C Meyer, B Schneider, S Jakob, S Strehl, A Attarbaschi, S Schnittger, C Schoch… in Leukemia (2006)

Article

Common genetic variants in the interleukin-6 and chitotriosidase genes are associated with the risk for serious infection in children undergoing therapy for acute myeloid leukemia

Infectious complications represent a substantial cause of morbidity and mortality in children undergoing therapy for acute myeloid leukemia (AML). Since it has been shown that alterations in innate immune path...

T Lehrnbecher, T Bernig, M Hanisch, U Koehl, M Behl, D Reinhardt, U Creutzig… in Leukemia (2005)

Article

Hourly monitoring of circulating CD34+ cells to optimize timing of autologous apheresis in pediatric patients

In order to increase the CD34+ cell yield in children undergoing autologous stem cell transplantation, the optimum time of apheresis after G-CSF administration has still to be found. We prospectively studied t...

K Bochennek, C Andreas, C Margraf, H Stahlkamp, S Zimmermann… in Bone Marrow Transplantation (2005)

Article

Use of natural killer cells in hematopoetic stem cell transplantation

Adoptive immunotherapy using natural killer (NK) cells may prove useful, especially in situations where infusion of T cells is impractical such as in recipients of haploidentical stem cell transplantation (HSC...

J R Passweg, M Stern, U Koehl, L Uharek, A Tichelli in Bone Marrow Transplantation (2005)

Article

Meningitis Due to Multiple-Resistant Penicillin- and Cefotaxime-Intermediate Streptococcus pneumoniae in a German Child after Bone Marrow Transplantation

The incidence of infection with penicillin-non-susceptible Streptococcus pneumoniae is increasing rapidly worldwide. Spain and France are highly affected, whereas the level of penicillin resistance in Germany, It...

H. Buxmann, J. Soerensen, U. Koehl, D. Schwabe, T. Klingebiel, R. R. Reinert… in Infection (2003)

Article

Kinetics of cell death in T lymphocytes genetically modified with two novel suicide fusion genes

Donor lymphocyte infusions (DLI) following allogeneic stem cell transplantation are known to mediate graft-versus-leukemia effect (GVL). A major side effect of these immunotherapies is the development of graft...

K Junker, U Koehl, S Zimmerman, S Stein, D Schwabe, T Klingebiel, M Grez in Gene Therapy (2003)

Article

Quantitative analysis of chimerism after allogeneic stem cell transplantation by PCR amplification of microsatellite markers and capillary electrophoresis with fluorescence detection: the Frankfurt experience

U Koehl, O Beck, R Esser, E Seifried, T Klingebiel, D Schwabe, C Seidl in Leukemia (2003)

Article

Autologous transplantation of CD133 selected hematopoietic progenitor cells in a pediatric patient with relapsed leukemia

A pediatric patient with very early meningeal relapse of his CD34⁺ CD133⁻ pre-B-ALL was transplanted with 2.5 × 10⁶/kg CD133 selected autologous progenitor cells. Enrichment of CD133⁺ cells resulted in a purity o...

U Koehl, S Zimmermann, R Esser, J Sörensen, HP Grüttner… in Bone Marrow Transplantation (2002)

Article

Tecelac as antithymocyte globulin in conditioning for childhood allogeneic stem cell transplantation

Antithymocyte globulin (ATG) preparations in allogeneic stem cell transplantation are used in various conditioning regimens both to prevent graft rejection and reduce the incidence and severity of graft-versus...

SY Zimmermann, T Klingebiel, U Koehl, J Soerensen, D Schwabe in Bone Marrow Transplantation (2002)