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Open AccessDevelo** centrifugal force real-time digital PCR for detecting extremely low DNA concentration
Digital PCR (dPCR) is a technique for absolute quantification of nucleic acid molecules. To develop a dPCR technique that enables more accurate nucleic acid detection and quantification, we established a novel...
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Article
Open AccessThe secreted protein Amuc_1409 from Akkermansia muciniphila improves gut health through intestinal stem cell regulation
Akkermansia muciniphila has received great attention because of its beneficial roles in gut health by regulating gut immunity, promoting intestinal epithelial development, and improving barrier integrity. However...
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Article
Open AccessTRPV1 inhibition overcomes cisplatin resistance by blocking autophagy-mediated hyperactivation of EGFR signaling pathway
Cisplatin resistance along with chemotherapy-induced neuropathic pain is an important cause of treatment failure for many cancer types and represents an unmet clinical need. Therefore, future studies should pr...
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Article
Open AccessNANOG confers resistance to complement-dependent cytotoxicity in immune-edited tumor cells through up-regulating CD59
Cancer immunoediting drives the adaptation of tumor cells to host immune surveillance. Previously, we have demonstrated that immunoediting driven by cytotoxic T lymphocytes (CTLs) enriches NANOG+ tumor cells with...
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Open AccessAuthor Correction: Deep learning method for comet segmentation and comet assay image analysis
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Open AccessDeep learning method for comet segmentation and comet assay image analysis
Comet assay is a widely used method, especially in the field of genotoxicity, to quantify and measure DNA damage visually at the level of individual cells with high sensitivity and efficiency. Generally, compu...
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Article
The CH25H–CYP7B1–RORα axis of cholesterol metabolism regulates osteoarthritis
Osteoarthritis—the most common form of age-related degenerative whole-joint disease1—is primarily characterized by cartilage destruction, as well as by synovial inflammation, osteophyte formation and subchondral ...
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Open AccessRNA-binding protein ZFP36L1 regulates osteoarthritis by modulating members of the heat shock protein 70 family
Osteoarthritis (OA) is a whole-joint disease characterized by cartilage destruction and other whole-joint pathological changes. There is currently no effective disease-modifying therapy. Here we investigate th...
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Open AccessThe impact of Arctic sea ice loss on mid-Holocene climate
Mid-Holocene climate was characterized by strong summer solar heating that decreased Arctic sea ice cover. Motivated by recent studies identifying Arctic sea ice loss as a key driver of future climate change, ...
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Open AccessUtility of targeted deep sequencing for detecting circulating tumor DNA in pancreatic cancer patients
Targeted deep sequencing across broad genomic regions has been used to detect circulating tumor DNA (ctDNA) in pancreatic ductal adenocarcinoma (PDAC) patients. However, since most PDACs harbor a mutation in K...
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Open AccessKisspeptin-10 (KP-10) stimulates osteoblast differentiation through GPR54-mediated regulation of BMP2 expression and activation
Kisspeptin-10 (KP-10) acts as a tumor metastasis suppressor via its receptor, G-protein-coupled receptor 54 (GPR54). The KP-10-GPR54 system plays an important role in embryonic kidney development. However, its...
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Open AccessVertical Magnetic Separation of Circulating Tumor Cells for Somatic Genomic-Alteration Analysis in Lung Cancer Patients
Efficient isolation and genetic analysis of circulating tumor cells (CTCs) from cancer patients’ blood is a critical step for clinical applications using CTCs. Here, we report a novel CTC-isolation method and ...
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Open AccessThe minimal amount of starting DNA for Agilent’s hybrid capture-based targeted massively parallel sequencing
Targeted capture massively parallel sequencing is increasingly being used in clinical settings, and as costs continue to decline, use of this technology may become routine in health care. However, a limited am...
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Open AccessHighly sensitive and selective sugar detection by terahertz nano-antennas
Molecular recognition and discrimination of carbohydrates are important because carbohydrates perform essential roles in most living organisms for energy metabolism and cell-to-cell communication. Nevertheless...