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Chapter
Iron-Sulfur Proteins: Investigations of Hyperfine-Shifted Hydrogen, Carbon, and Nitrogen Resonances
Iron-sulfur proteins are present in almost all living organisms. They are characterized by one or more iron ions ligated to inorganic sulfur and/or cysteine sulfur. Rubredoxin-type clusters contain a single ir...
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Chapter
NMR Approaches to the Study of Structure-Function Relationships in Iron-Sulfur Proteins
Newer NMR methods, particularly in conjunction with stable isotope labeling (with 2H, 13C, and 15N), offer powerful approaches to the elucidation of structure-function relationships in paramagnetic proteins such ...
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Article
Structural and kinetic characterization of early folding events in β-lactoglobulin
We have defined the structural and dynamic properties of an early folding intermediate of β-lactoglobulin known to contain non-native α-helical structure. The folding of β-lactoglobulin was monitored over the ...
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Article
Electroantennogram responses of Dendrolimus superans (Lepidoptera: Lasiocampidae) to six volatiles of Larix gmelinii
Dendrolimus superans is one of the important pests feeding on the needles of Larix gmelinii. Six standard compounds, (1R)-(−)-α-pinene, (1S)-(−)-α-pinene, ocimene, (1S)-(−)-β-pinene, R(−)...
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Article
The low temperature-responsive, Solanum CBF1 genes maintain high identity in their upstream regions in a genomic environment undergoing gene duplications, deletions, and rearrangements
Some plants like Arabidopsis thaliana increase in freezing tolerance when exposed to low nonfreezing temperatures, a process known as cold acclimation. Other plants including tomato, Solanum lycopersicum, are chi...
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Article
Changes in extreme high-temperature tolerance and activities of antioxidant enzymes of sacred lotus seeds
Sacred lotus (Nelumbo nucifera Gaertn. ‘Tielian’) seed is long-lived and extremely tolerant of high temperature. Water content of lotus and maize seeds was 0.103 and 0.129 g H2O [g DW] −1, respectively. Water con...
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Article
Open AccessExploring transcriptional signalling mediated by OsWRKY13, a potential regulator of multiple physiological processes in rice
Rice transcription regulator OsWRKY13 influences the functioning of more than 500 genes in multiple signalling pathways, with roles in disease resistance, redox homeostasis, abiotic stress responses, and devel...
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Article
Molecular cloning, characterization and expression analysis of CmAPX
The RT PCR and RACE methods were used to obtain the cDNA sequence of an APX gene of muskmelon after the leaves were induced with powdery mildew. The cDNA length of the APX gene is 1,047 bp with a 750 bp ORF en...
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Article
Isolation and characterization of a gene encoding cinnamate 4-hydroxylase from Parthenocissus henryana
Cinnamate 4-hydroxylase (C4H, EC 1.14.13.11) plays an important role in the phenylpropanoid pathway, which produces many economically important secondary metabolites. A gene coding for C4H, designated as PhC4H (G...
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Chapter
Discriminative Frequent Pattern-Based Graph Classification
Frequent graph mining has been studied extensively with many scalable graph mining algorithms developed in the past. Graph patterns are essential not only for exploratory graph mining but also for advanced gra...
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Article
Cytological and physiological changes in orthodox maize embryos during cryopreservation
Cytological and physiological changes during cryopreservation were studied in maize embryos at 35 days after pollination (DAP). Both dehydration and freezing caused cytological damage, such as plasmolysis, swe...
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Article
CBF gene copy number variation at Frost Resistance-2 is associated with levels of freezing tolerance in temperate-climate cereals
Frost Resistance-1 (FR-1) and FR-2 are two loci affecting freezing tolerance and winter hardiness of the temperate-climate cereals. FR-1 is hypothesized to be due to the pleiotropic effects of VRN-1. FR-2 spans a...
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Article
Agrobacterium-mediated transformation of Eupatorium adenophorum
A simple and efficient protocol for Agrobacterium tumefaciens-mediated genetic transformation of Eupatorium adenophorum has been developed. Stem segments were pre-cultured on Murashige and Skoog (MS) medium for 2...
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Article
Notch-induced Asb2 expression promotes protein ubiquitination by forming non-canonical E3 ligase complexes
Notch signaling controls multiple developmental processes, thus demanding versatile functions. We have previously shown that this may be partly achieved by accelerating ubiquitin-mediated degradation of import...
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Article
Nitrite, sodium nitroprusside, potassium ferricyanide and hydrogen peroxide release dormancy of Amaranthus retroflexus seeds in a nitric oxide-dependent manner
Nitric oxide (NO) and reactive oxygen species (ROS) are important regulators involving various processes of plant growth and development. Amaranthus retroflexus L. seeds possess a relative dormancy property that ...
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Article
Molecular cloning of allelopathy related genes and their relation to HHO in Eupatorium adenophorum
In this study, conserved sequence regions of HMGR, DXR, and CHS (encoding 3-hydroxy-3-methylglutaryl-CoA reductase, 1-deoxyxylulose-5-phosphate reductoisomerase and chalcone synthase, respectively) were amplified...
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Article
Novel human BTB/POZ domain-containing zinc finger protein ZBTB1 inhibits transcriptional activities of CRE
BTB/POZ protein family plays a key role in many biological processes by regulating the transcriptional activities of some downstream genes. Here, we characterized the member of C2H2 type zinc finger gene, Zinc fi...
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Article
Molecular cloning and expression analysis of CmMlo1 in melon
Mlo gene encodes an important transmembrane protein that is involved in biotic/abiotic stresses. Using the method of homologous, we cloned a Mlo gene from melon, named CmMlo1. The gene is...
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Article
Nitrate, abscisic acid and gibberellin interactions on the thermoinhibition of lettuce seed germination
Germination of lettuce seeds has obvious thermoinhibition, but the mechanism for thermoinhibition of seed germination is poorly understood. Here, we investigated the interactions of nitrate, abscisic acid (ABA...
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Article
Activation of transcriptional activity of HSE by a novel mouse zinc finger protein ZNFD specifically expressed in testis
Zinc finger proteins (ZFPs) that contain multiple cysteine and/or histidine residues perform important roles in various cellular functions, including transcriptional regulation, cell proliferation, differentia...