24 Result(s)

Article

Identification and Evaluation of Reference Genes for qRT-PCR Normalization in Ganoderma lucidum

Quantitative real-time reverse transcription PCR (qRT-PCR) is a rapid, sensitive, and reliable technique for gene expression studies. The accuracy and reliability of qRT-PCR results depend on the stability of ...

Jiang Xu, ZhiChao Xu, YingJie Zhu, HongMei Luo, Jun Qian, AiJia Ji… in Current Microbiology (2014)

Article

Expression of SYMRK affects the development of arbuscular mycorrhiza in tobacco roots

SYMRK is a plant receptor-like kinase with a role in root endosymbiosis. Heterologous expression of SYMRK from non-legumes can complement the loss-of-function effects of the mutant symrk in legumes. However, it i...

Zhi**g Tan, Yuanlei Hu, Zhong** Lin in Acta Physiologiae Plantarum (2013)

Article

PhPT4 Is a Mycorrhizal-Phosphate Transporter Suppressed by Lysophosphatidylcholine in Petunia Roots

An important characteristic of arbuscular mycorrhizal (AM) symbiosis is the transfer of phosphate from AM fungi to plants. This transfer is facilitated by mycorrhiza-inducible phosphate transporter proteins. L...

Zhi**g Tan, Yuanlei Hu, Zhong** Lin in Plant Molecular Biology Reporter (2012)

Article

Production of isoflavone genistein in transgenic IFS tobacco roots and its role in stimulating the development of arbuscular mycorrhiza

Flavonoids and isoflavonoids are secondary metabolites in plants. With the goal of obtaining isoflavonoids from a wide range of plants, a few key studies have proven that isoflavonoids can be produced in non-l...

Zhi**g Tan, Rongrong Liu, Yuanlei Hu, Zhong** Lin in Acta Physiologiae Plantarum (2012)

Article

Expression of NtPT5 Is Correlated with the Degree of Colonization in Tobacco Roots Inoculated with Glomus etunicatum

An important characteristic of arbuscular mycorrhizal (AM) symbiosis is the transfer of phosphate (Pi) from AM fungi to plants, and this transfer is facilitated by plasma membrane-spanning phosphate transporte...

Zhi**g Tan, Yuanlei Hu, Zhong** Lin in Plant Molecular Biology Reporter (2012)

Article

Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio)

Aquaculture farming may benefit from genetically engineering fish to tolerate environmental stress. Here, we used the vector pCVCG expressing the Vitreoscilla hemoglobin (vhb) gene driven by the common carp β-act...

Bo Guan, Hong Ma, Ya** Wang, Yuanlei Hu, Zhong** Lin… in Marine Biotechnology (2011)

Article

Isolation and characterization of a gene encoding cinnamate 4-hydroxylase from Parthenocissus henryana

Cinnamate 4-hydroxylase (C4H, EC 1.14.13.11) plays an important role in the phenylpropanoid pathway, which produces many economically important secondary metabolites. A gene coding for C4H, designated as PhC4H (G...

Shujun Liu, Yuanlei Hu, **aoli Wang, Li Han, Songquan Song… in Molecular Biology Reports (2009)

Article

Molecular cloning and characterization of a gene encoding RING zinc finger ankyrin protein from drought-tolerant Artemisia desertorum

A RING zinc finger ankyrin protein gene, designated AdZFP1, was isolated from drought-tolerant Artemisia desertorum Spreng by mRNA differential display and RACE. Its cDNA was 1723 bp and encoded a putative protei...

**uhong Yang, Chao Sun, Yuanlei Hu, Zhong** Lin in Journal of Biosciences (2008)

Article

Expression profile of a PAL gene from Astragalus membranaceus var. Mongholicus and its crucial role in flux into flavonoid biosynthesis

An important traditional Chinese medicine herb, Astragalus membranaceus var. Mongholicus, whose dried root is known as Radix astragali (“Huangqi” in Chinese), has high flavonoid content as an essential active con...

Rongrong Liu, Shaohua Xu, Jialin Li, Yuanlei Hu, Zhong** Lin in Plant Cell Reports (2006)

Article

Inducible Excision of Selectable Marker Gene from Transgenic Plants by the Cre/lox Site-specific Recombination System

In a plant transformation process, it is necessary to use marker genes that allow the selection of regenerated transgenic plants. However, selectable marker genes are generally superfluous once an intact trans...

Yong Wang, Bojun Chen, Yuanlei Hu, **gfu Li, Zhong** Lin in Transgenic Research (2005)

Article

Transgenic tall fescue containing the Agrobacterium tumefaciens ipt gene shows enhanced cold tolerance

Embryogenic calli of Festuca arundinacea were transformed with the Agrobacterium tumefaciens isopentenyl transferase (ipt) gene driven by a maize ubiquitin promoter. Tillering ability, levels of chlorophyll a and

Yuanlei Hu, Weilong Jia, Jundan Wang, Yanqin Zhang, Lili Yang… in Plant Cell Reports (2005)

Article

Anchored PCR (A-PCR): A new method for chromosome walking

Bojun Chen, Chao Sun, Yong Wang, Yuanlei Hu, Zhong** Lin in Chinese Science Bulletin (2004)

Article

Ectopic expression of a c1-I allele from maize inhibits pigment formation in the flower of transgenic tobacco

MYB-related proteins play a key role in regulating the biosynthesis of anthocyanins in plants at the transcriptional level. An MYB gene designated cl-I-2K1 (GenBank accession no. AY237128) was cloned from maize l...

Bojun Chen, **qing Wang, Yuanlei Hu, Yong Wang, Zhong** Lin in Molecular Biotechnology (2004)

Article

Allergenicity assay of allergen fromDermatophagoides farinae in transgenic tobacco

Derf ₂ gene for one of mite allergens inDermatophagoides farinae has been cloned and expressed under regulation of 35S promoter...

Mingjuan Tang, Ye Shen, Yuanlei Hu, Lei Cao, Ting Ni… in Chinese Science Bulletin (2004)

Article

Cloning of the promoter region of plasma membrane aquaporinBnPIP1 fromBrassica napus and its functional analysis

A 1.6 kb upstream regulatory sequence (GenBank accession no. AF472487) of plasma membrane aquaporinBnPIP1 gene fromBrassica napus was obtained by genomic walking based on ligation-mediated PCR method. Sequence an...

Qiuju Yu, Li Du, Yuanlei Hu, Zhong** Lin in Science in China Series C: Life Sciences (2003)

Article

Promoter of the rolC gene of Agrobacterium rhizogenes can be strongly regulated in glandular cell of transgenic tobacco

A 577-bp promoter segment of Agrobacterium rhizogenes rolC, previously known as the phloem-specific gene expression promoter, was fused to the 5′ end of a reporter gene, β-glucuronidase (GUS), uidA. This rolC-pro...

Yuanlei Hu, Bojun Chen, Ting Ni, Ning Li, Zhong** Lin in Molecular Biotechnology (2003)

Article

Expression of ipt gene driven by tomato fruit specific promoter and its effects on fruit development of tomato

Fruit specific promoter (2A12) from Lycopersicom esculentum and cDNA of isopentenyl-transferase (ipt) from Ti plasmid of Agrobacterium tumerfaciens C58 were cloned by PCR procedure respectively. Two plant express...

Zichao Mao, Qiuju Yu, Wei Zhen, Junyi Guo, Yuanlei Hu, Yin Gao… in Chinese Science Bulletin (2002)

Article

CaMV 35S promoter directs β-glucuronidase expression in Ganoderma lucidum and Pleurotus citrinopileatus

The cauliflower mosaic virus (CaMV) 35S promoter has been most commonly used in plant transformation studies, but its activity in mushrooms has not been reported. p301-b is a binary vector containing a bialaph...

Li Sun, Huaqing Cai, Weihong Xu, Yuanlei Hu, Zhong** Lin in Molecular Biotechnology (2002)

Article

Efficient transformation of the medicinal mushroomGanoderma lucidum

Ganoderma lucidum is a well-known and important medicinal mushroom, but its genetic modification has not been reported. We developed an efficient procedure for isolation and regeneration of proto...

Li Sun, Huaqing Cai, Weihong Xu, Yuanlei Hu, Yin Gao… in Plant Molecular Biology Reporter (2001)