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Protocol
Identification of Subproteomic Markers for Skeletal Muscle Profiling
The biochemical and cell biological profiling of contractile fiber types and subcellular structures plays a central role in basic and applied myology. Mass spectrometry-based proteomics presents an ideal appro...
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Protocol
Histological and Histochemical Microscopy Used to Verify 2D-DIGE Pathoproteomics
Comparative gel electrophoretic analyses of normal versus pathological specimens can swiftly identify proteome-wide changes in the concentration of specific protein isoforms. The application of fluorescence tw...
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Protocol
Proteomic Identification of Saliva Proteins as Noninvasive Diagnostic Biomarkers
Many biomedically relevant biomarkers are proteins with characteristic biochemical properties and a relatively restricted subcellular distribution. The comparative and mass spectrometry-based proteomic analysi...
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Protocol
Bioinformatic Analysis of the Subproteomic Profile of Cardiomyopathic Tissue
Following large-scale protein separation by two-dimensional gel electrophoresis or liquid chromatography, mass spectrometry–based proteomics can be used for the swift identification and characterization of car...
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Protocol
Verification of Protein Changes Determined by 2D-DIGE Based Proteomics Using Immunofluorescence Microscopy
Fluorescence two-dimensional difference gel electrophoresis (2D-DIGE) is a key biochemical method for the comparative analysis of complex protein mixtures. The technique focuses on the identification and chara...
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Article
Open AccessComplexity of skeletal muscle degeneration: multi-systems pathophysiology and organ crosstalk in dystrophinopathy
Duchenne muscular dystrophy is a highly progressive muscle wasting disorder due to primary abnormalities in one of the largest genes in the human genome, the DMD gene, which encodes various tissue-specific isofor...
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Article
Proteomic profiling of the mouse diaphragm and refined mass spectrometric analysis of the dystrophic phenotype
The diaphragm is a crucial muscle involved in active inspiration and whole body homeostasis. Previous biochemical, immunochemical and cell biological investigations have established the distribution and fibre ...
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Article
Open AccessProteomic profiling of liver tissue from the mdx-4cv mouse model of Duchenne muscular dystrophy
Duchenne muscular dystrophy is a highly complex multi-system disease caused by primary abnormalities in the membrane cytoskeletal protein dystrophin. Besides progressive skeletal muscle degeneration, this neur...
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Protocol
Immunofluorescence Microscopy for DIGE-Based Proteomics
Alterations in the proteome of a tissue in different settings, as assessed by difference gel electrophoresis, can be verified for single proteins using immunohistochemistry. In fluorescence immunohistochemistr...
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Article
Open AccessSphingosine 1-phosphate lyase ablation disrupts presynaptic architecture and function via an ubiquitin- proteasome mediated mechanism
The bioactive lipid sphingosine 1-phosphate (S1P) is a degradation product of sphingolipids that are particularly abundant in neurons. We have shown previously that neuronal S1P accumulation is toxic leading t...
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Article
Open AccessLabel-free mass spectrometric analysis reveals complex changes in the brain proteome from the mdx-4cv mouse model of Duchenne muscular dystrophy
X-linked muscular dystrophy is a primary disease of the neuromuscular system. Primary abnormalities in the Dmd gene result in the absence of the full-length isoform of the membrane cytoskeletal protein dystrophin...
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Article
Open AccessErratum to: The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation
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Article
Expression of early hippocampal CA1 LTP does not lead to changes in AMPA-EPSC kinetics or sensitivity to cyclothiazide
We have analysed whether the expression of long-term potentiation (LTP) in rat hippocampal CA1 neurons involves a change in the kinetics of (S)-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) recepto...
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Chapter
Calcium Regulation of Ion Channels
It is well established that intracellular Ca ions modulate cellular membrane potential and thus excitability by affecting the gating of ion channels in the cell membrane. One of the targets for Ca ions are Ca2+ c...
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Article
Calcium channel types contributing to excitatory and inhibitory synaptic transmission between individual hypothalamic neurons
The contribution of L-, N-, P- and Q-type Ca2+ channels to excitatory and inhibitory synaptic transmission and to whole-cell Ba2+ currents through Ca2+ channels (Ba2+ currents) was investigated in rat hypothalami...
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Article
Calcium buffering in bursting Helix pacemaker neurons
Bursting pacemaker neurons of the snail Helix pomatia were voltage-clamped and Ca currents in response to depolarizing steps were recorded. Simultaneously, changes in intracellular Ca concentrations were measured...
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Chapter
Control of Cell Function by Neuronal Calcium-Activated Nonselective (CAN) Cation Channels
Much of the action of the nervous system can be ultimately associated with changes in the potential of individual neurons. Inhibition plays an essential role in neuronal interactions but it is the excitation p...
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Article
A diacylglycerol analogue reduces neuronal calcium currents independently of protein kinase C activation
DIACYLGLYCEROL analogues (for example 1,2-oleoylacetyl-glycerol, OAG) and phorbol esters are activators of protein kinase C, and have been widely used to study the function of this enzyme in both intact cells ...
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Article
Direct ion channel gating: A new function for intracellular messengers
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There is widespread belief that intracellular messengers [e.g., Ca2+, cyclic AMP, cyclic GMP, inositol-1,4,5-...
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