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Article
Open AccessComparing the efficacy in reducing brain injury of different neuroprotective agents following neonatal hypoxia–ischemia in newborn rats: a multi-drug randomized controlled screening trial
Intrapartum hypoxia–ischemia leading to neonatal encephalopathy (NE) results in significant neonatal mortality and morbidity worldwide, with > 85% of cases occurring in low- and middle-income countries (LMIC)....
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Protocol
Histological and Histochemical Microscopy Used to Verify 2D-DIGE Pathoproteomics
Comparative gel electrophoretic analyses of normal versus pathological specimens can swiftly identify proteome-wide changes in the concentration of specific protein isoforms. The application of fluorescence tw...
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Protocol
Bioinformatic Analysis of the Subproteomic Profile of Cardiomyopathic Tissue
Following large-scale protein separation by two-dimensional gel electrophoresis or liquid chromatography, mass spectrometry–based proteomics can be used for the swift identification and characterization of car...
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Protocol
Verification of Protein Changes Determined by 2D-DIGE Based Proteomics Using Immunofluorescence Microscopy
Fluorescence two-dimensional difference gel electrophoresis (2D-DIGE) is a key biochemical method for the comparative analysis of complex protein mixtures. The technique focuses on the identification and chara...
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Article
Proteomic profiling of the mouse diaphragm and refined mass spectrometric analysis of the dystrophic phenotype
The diaphragm is a crucial muscle involved in active inspiration and whole body homeostasis. Previous biochemical, immunochemical and cell biological investigations have established the distribution and fibre ...
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Article
Open AccessProteomic profiling of liver tissue from the mdx-4cv mouse model of Duchenne muscular dystrophy
Duchenne muscular dystrophy is a highly complex multi-system disease caused by primary abnormalities in the membrane cytoskeletal protein dystrophin. Besides progressive skeletal muscle degeneration, this neur...
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Protocol
Immunofluorescence Microscopy for DIGE-Based Proteomics
Alterations in the proteome of a tissue in different settings, as assessed by difference gel electrophoresis, can be verified for single proteins using immunohistochemistry. In fluorescence immunohistochemistr...
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Article
Open AccessLabel-free mass spectrometric analysis reveals complex changes in the brain proteome from the mdx-4cv mouse model of Duchenne muscular dystrophy
X-linked muscular dystrophy is a primary disease of the neuromuscular system. Primary abnormalities in the Dmd gene result in the absence of the full-length isoform of the membrane cytoskeletal protein dystrophin...
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Article
Contractile properties, structure and fiber phenotype of intact and regenerating slow-twitch muscles of mice treated with cyclosporin A
We have studied the contractile properties, structure, fiber-type composition, and myosin heavy chain (MyHC) expression pattern of regenerating and intact soleus muscles of adult CBA/J mice treated with cyclo...
