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Chapter
Mathematics Education: Is It Ethical?
In this chapter, I want to ask the question, ‘is mathematics education as practiced, primarily in schools, an ethical activity?’ I shall illustrate the point of my question by reference to several cases, inclu...
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Article
Open AccessCorrection: PP2A inhibition overcomes acquired resistance to HER2 targeted therapy
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Protocol
DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-Fixed Paraffin-Embedded (FFPE) Tissue
In this chapter, we describe the utility of fluorescence two-dimensional difference gel electrophoresis (2D-DIGE) as a proteomics platform for the global detection of expressed proteins in formalin-fixed paraf...
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Protocol
Analysis of the Saliva Proteome Using 2D-DIGE
Saliva is now an established biofluid with a number of important applications in use across research and clinical settings. Saliva contains an assortment of biomolecules, including proteins, metabolites, RNA, ...
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Protocol
DIGE Analysis of Immunodepleted Plasma
This chapter focuses on upstream immunodepletion of high-abundance proteins from plasma samples and subsequent analysis by fluorescence two-dimensional difference gel electrophoresis (2D-DIGE). The abundances ...
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Protocol
Analysis of Cancer Cell Line Secretomes: A Complementary Source of Disease-Specific Protein Biomarkers
Various types of cancer cells enrich or condition the medium that they are cultured in by secreting or shedding proteins and small molecules. These secreted or shed factors are involved in key biological proce...
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Protocol
DIGE-Based Biomarker Discovery in Blood Cancers
Cancer of blood or bone marrow-derived cells dysregulates normal hematopoiesis and accounts for over 6% of all cancer cases annually. Proteomic analyses of blood cancers have improved our understanding of dise...
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Protocol
DIGE Analysis of ProteoMiner™ Fractionated Serum/Plasma Samples
The discovery of clinically relevant biomarkers using gel-based proteomics has proven extremely challenging, principally because of the large dynamic range of protein abundances in biofluids such as blood and ...
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Protocol
Identification of Subproteomic Markers for Skeletal Muscle Profiling
The biochemical and cell biological profiling of contractile fiber types and subcellular structures plays a central role in basic and applied myology. Mass spectrometry-based proteomics presents an ideal appro...
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Protocol
DIGE Analysis Software and Protein Identification Approaches
Two-dimensional difference gel electrophoresis (2D-DIGE) is a high-resolution protein separation technique, with the excellent dynamic range obtained by fluorescent tag labeling of protein samples. Scanned ima...
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Protocol
Identification of Ubiquitination-Associated Proteins Using 2D-DIGE
Ubiquitination is a post-translational modification, in which a small regulatory protein (~8.6 kDa) is tagged as a single moiety or as a chain to target proteins. Ubiquitination is the most versatile cellular ...
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Protocol
Proteomic Identification of Saliva Proteins as Noninvasive Diagnostic Biomarkers
Many biomedically relevant biomarkers are proteins with characteristic biochemical properties and a relatively restricted subcellular distribution. The comparative and mass spectrometry-based proteomic analysi...
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Article
Open AccessNext generation proteomics with drug sensitivity screening identifies sub-clones informing therapeutic and drug development strategies for multiple myeloma patients
With the introduction of novel therapeutic agents, survival in Multiple Myeloma (MM) has increased in recent years. However, drug-resistant clones inevitably arise and lead to disease progression and death. Th...
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Reference Work Entry In depth
Recontextualization in Mathematics Education
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Article
Open AccessTargeting histone deacetylase 3 (HDAC3) in the bone marrow microenvironment inhibits multiple myeloma proliferation by modulating exosomes and IL-6 trans-signaling
Multiple myeloma (MM) is an incurable cancer that derives pro-survival/proliferative signals from the bone marrow (BM) niche. Novel agents targeting not only cancer cells, but also the BM-niche have shown the ...
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Article
Open AccessDevelopment and characterisation of a panel of phosphatidylinositide 3-kinase – mammalian target of rapamycin inhibitor resistant lung cancer cell lines
The PI3K-mTOR pathway is involved in regulating all hallmarks of cancer, and is often dysregulated in NSCLC, making it an attractive therapeutic target in this setting. Acquired resistance to PI3K-mTOR inhibit...
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Protocol
DIGE Analysis of Immunodepleted Plasma
This chapter will focus on upstream immunodepletion of high abundant proteins from plasma samples and subsequent analysis by difference gel electrophoresis (DIGE). The abundances of proteins in biofluid proteo...
-
Protocol
DIGE Analysis of ProteoMinerTM Fractionated Serum/Plasma Samples
The discovery of clinically relevant biomarkers using gel-based proteomics has proven extremely challenging, principally because of the large dynamic range of protein abundances in biofluids such as blood and ...
-
Protocol
DIGE Analysis Software and Protein Identification Approaches
DIGE is a high-resolution two-dimensional gel electrophoresis method, with excellent dynamic range obtained by fluorescent tag labeling of protein samples. Scanned images of DIGE gels show thousands of protein...
-
Protocol
DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-Fixed Paraffin-Embedded (FFPE) Tissue
In this chapter, we describe the utility of difference gel electrophoresis (DIGE) as a proteomics platform for the global detection of expressed proteins in formalin-fixed paraffin-embedded (FFPE) tissues and ...
