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Article
Open AccessA newly identified gene Ahed plays essential roles in murine haematopoiesis
The development of haematopoiesis involves the coordinated action of numerous genes, some of which are implicated in haematological malignancies. However, the biological function of many genes remains elusive ...
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Article
Periportal macrophages protect against commensal-driven liver inflammation
The liver is the main gateway from the gut, and the unidirectional sinusoidal flow from portal to central veins constitutes heterogenous zones, including the periportal vein (PV) and the pericentral vein zones1–5
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Article
Open AccessMultiple ageing effects on testicular/epididymal germ cells lead to decreased male fertility in mice
In mammals, females undergo reproductive cessation with age, whereas male fertility gradually declines but persists almost throughout life. However, the detailed effects of ageing on germ cells during and afte...
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Article
Open AccessSingle-cell transcriptomics of human cholesteatoma identifies an activin A-producing osteoclastogenic fibroblast subset inducing bone destruction
Cholesteatoma, which potentially results from tympanic membrane retraction, is characterized by intractable local bone erosion and subsequent hearing loss and brain abscess formation. However, the pathophysiol...
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Article
Open AccessWhole-blood ribonucleic acid sequencing analysis in methemoglobinemia: a case report
Methemoglobinemia is a condition in which methemoglobin is increased and the oxygen carrying capacity of tissues is decreased, causing a lack of oxygen to the whole body. RNA (ribonucleic acid) sequencing tech...
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Article
Open AccessSingle-cell analyses and host genetics highlight the role of innate immune cells in COVID-19 severity
Mechanisms underpinning the dysfunctional immune response in severe acute respiratory syndrome coronavirus 2 infection are elusive. We analyzed single-cell transcriptomes and T and B cell receptors (BCR) of >8...
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Article
Open AccessCancer-specific tissue-resident memory T-cells express ZNF683 in colorectal cancer
Tissue-resident memory T (Trm) cells are associated with cytotoxicity not only in viral infection and autoimmune disease pathologies but also in many cancers. Tumour-infiltrating CD103+ Trm cells predominantly co...
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Article
Open AccessJAK inhibition ameliorates bone destruction by simultaneously targeting mature osteoclasts and their precursors
Rheumatoid arthritis (RA) is characterized by chronic inflammation and resultant cartilage/bone destruction because of aberrantly activated osteoclasts. Recently, novel treatments with several Janus kinase (JA...
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Article
Open AccessCombination of WFDC2, CHI3L1, and KRT19 in Plasma Defines a Clinically Useful Molecular Phenotype Associated with Prognosis in Critically Ill COVID-19 Patients
COVID-19 is now a common disease, but its pathogenesis remains unknown. Blood circulating proteins reflect host defenses against COVID-19. We investigated whether evaluation of longitudinal blood proteomics fo...
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Article
Open AccessIn vivo induction of activin A-producing alveolar macrophages supports the progression of lung cell carcinoma
Alveolar macrophages (AMs) are crucial for maintaining normal lung function. They are abundant in lung cancer tissues, but their pathophysiological significance remains unknown. Here we show, using an orthotop...
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Unfolding the Bacterial Transcriptome Landscape Using Oxford Nanopore Technology Direct RNA Sequencing
Current genome annotation ignores important features of the transcriptome, such as untranslated regions and operon maps. RNA sequencing (RNA-seq) helps in identifying such features; however, the fragmentation ...
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Article
Open AccessTranscriptional differences between coronavirus disease 2019 and bacterial sepsis
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2, has led to major public health crises worldwide. Several studies have reported the comprehensive mRNA expression ana...
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Article
Open AccessSymbiotic bacteria-dependent expansion of MR1-reactive T cells causes autoimmunity in the absence of Bcl11b
MHC class I-related protein 1 (MR1) is a metabolite-presenting molecule that restricts MR1-reactive T cells including mucosal-associated invariant T (MAIT) cells. In contrast to MAIT cells, the function of oth...
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Article
Food odor perception promotes systemic lipid utilization
Food cues during fasting elicit Pavlovian conditioning to adapt for anticipated food intake. However, whether the olfactory system is involved in metabolic adaptations remains elusive. Here we show that food-o...
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Article
Open AccessMyeloid cell-specific ablation of Runx2 gene exacerbates post-infarct cardiac remodeling
Runt-related transcription factor 2 (Runx2), a regulator of osteoblast differentiation, is pathologically involved in vascular calcification; however, the significance of Runx2 in cardiac homeostasis remains u...
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Article
Open AccessDOCK2 is involved in the host genetics and biology of severe COVID-19
Identifying the host genetic factors underlying severe COVID-19 is an emerging challenge1–5. Here we conducted a genome-wide association study (GWAS) involving 2,393 cases of COVID-19 in a cohort of Japanese indi...
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Article
Open AccessDevelopment of clinical phenotypes and biological profiles via proteomic analysis of trauma patients
Trauma is a heterogeneous condition, and specific clinical phenotypes may identify target populations that could benefit from certain treatment strategies. In this retrospective study, we determined clinical p...
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Article
Open AccessMyasthenia gravis-specific aberrant neuromuscular gene expression by medullary thymic epithelial cells in thymoma
Myasthenia gravis (MG) is a neurological disease caused by autoantibodies against neuromuscular-associated proteins. While MG frequently develops in thymoma patients, the etiologic factors for MG are not well ...
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Article
Open AccessImpaired differentiation potential of CD34-positive cells derived from mouse hair follicles after long-term culture
Hair follicle epithelial stem cells (HFSCs), which exist in the bulge region, have important functions for homeostasis of skin as well as hair follicle morphogenesis. Although several methods for isolation of ...
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Article
Open AccessCell response analysis in SARS-CoV-2 infected bronchial organoids
The development of an in vitro cell model that can be used to study severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) research is expected. Here we conducted infection experiments in bronchial organ...
