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  1. Article

    Open Access

    Impaired differentiation potential of CD34-positive cells derived from mouse hair follicles after long-term culture

    Hair follicle epithelial stem cells (HFSCs), which exist in the bulge region, have important functions for homeostasis of skin as well as hair follicle morphogenesis. Although several methods for isolation of ...

    Yukiteru Ouji, Masayasu Misu, Tomotaka Kitamura, Daisuke Okuzaki… in Scientific Reports (2022)

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  2. Article

    Open Access

    Ancylostoma ceylanicum, novel etiological agent for traveler’s diarrhea—report of four Japanese patients who returned from Southeast Asia and Papua New Guinea

    Countries in the Southeast Asia region have a high prevalence of soil-transmitted helminth, such as roundworm, whipworm, and hookworms [Ancylostoma duodenale, Necator americanus, Ancylostoma ceylanicum]. Recent m...

    Masahide Yoshikawa, Yukiteru Ouji, Nobuyasu Hirai… in Tropical Medicine and Health (2018)

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  3. No Access

    Article

    Antitumor effects of minodronate, a third-generation nitrogen-containing bisphosphonate, in synergy with γδT cells in human glioblastoma in vitro and in vivo

    Nitrogen-containing bisphosphonates (N-BPs), which prevent bone resorption, exert direct and γδT cell (GDT)-mediated antitumor effects against several tumor cell types, including glioblastoma (GBM). However, l...

    Tsutomu Nakazawa, Mitsutoshi Nakamura, Ryosuke Matsuda… in Journal of Neuro-Oncology (2016)

  4. No Access

    Protocol

    Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro

    CD49f+ CD34+ cells, a population rich in skin epithelial stem cells (EpSCs), were obtained from adult mouse skin and cultured with Wnt-3a for 10 days. On day 10, CD49f+ CD34+ cells were sorted and subjected to...

    Yukiteru Ouji, Masahide Yoshikawa in Stem Cell Heterogeneity (2016)

  5. No Access

    Protocol

    Induction of Inner Ear Hair Cells from Mouse Embryonic Stem Cells In Vitro

    Inner ear hair cells (HCs) function as the primary transducers for perception of sound and balance, while a defect in their formation or their loss results in sensory deficits. In mammals, once HCs are lost, t...

    Masahide Yoshikawa, Yukiteru Ouji in Stem Cell Heterogeneity (2016)

  6. No Access

    Protocol

    Maintenance of Dermal Papilla Cells by Wnt-10b In Vitro

    Dermal papilla cells (DPCs) are associated with development of hair follicles (HFs) and regulation of the hair cycle. However, primary DPCs are known to lose their ability to induce HFs after culture in standa...

    Yukiteru Ouji, Masahide Yoshikawa in Stem Cell Heterogeneity (2016)

  7. No Access

    Article

    Cytotoxic human peripheral blood-derived γδT cells kill glioblastoma cell lines: implications for cell-based immunotherapy for patients with glioblastoma

    Glioblastoma (GBM) is a highly aggressive brain tumor for which novel therapeutic approaches, such as immunotherapy, are urgently needed. Zoledronate (ZOL), an inhibitor of osteoclastic activity, is known to s...

    Tsutomu Nakazawa, Mitsutoshi Nakamura, Young Soo Park… in Journal of Neuro-Oncology (2014)

  8. No Access

    Protocol

    Derivation and Characterization of Hepatocytes From Embryonic Stem Cells In Vitro

    From a therapeutic perspective on liver, the use of embryonic stem (ES) technology in the generation of a large number of high-functional hepatocytes developed from ES cells for cell transplantation is anticip...

    Shigeaki Ishizaka, Yukiteru Ouji, Masahide Yoshikawa… in Embryonic Stem Cell Protocols (2006)

  9. No Access

    Article

    Capillary Electrophoresis with Chemiluminescent Detection for Luminol Using Potassium Ferricyanide as a Catalyst

    Kazuhiko Tsukagoshi, Yukiteru Ouji, Riichiro Nakajima in Analytical Sciences (2001)