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Article
Scale-up laccase production from Trametes versicolor stimulated by vanillic acid
An efficient strategy for laccase production in Trametes versicolor cultures was developed using vanillic acid as the inducer. The optimized vanillic acid treatment strategy consisted of exposing 2-day-old myceli...
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Article
Chitosan multiple addition enhances laccase production from Trametes versicolor
Chitosan multiple addition strategy was developed to improve laccase production from Trametes versicolor cultures. The optimized multiple addition strategy was carried out by two-time addition of 0.1 g L−1 chitos...
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Article
Development of an efficient process intensification strategy for enhancing Pfu DNA polymerase production in recombinant Escherichia coli
An efficient induction strategy that consisted of multiple additions of small doses of isopropyl-β-D-thiogalactopyranoside (IPTG) in the early cell growth phase was developed for enhancing Pfu DNA polymerase prod...
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Article
Efficient Kinetic Resolution of (R,S)-2-Octanol Catalyzed by Magnetite-Immobilized Yarrowia lipolytica Lipase in Mixed Ionic Liquids
The resolution of (R,S)-2-octanol catalyzed by magnetite-immobilized Yarrowia lipolytica lipase in ionic liquids was investigated. The optimal activity and enantioselectivity of the lipase were obtained in a mixt...
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Article
Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed
A magnetically stabilized fluidized bed (MSFB) with the Cu2+-chelated magnetic mesoporous silica nanoparticles (MMSNPs-Cu2+) was established to purify laccase directly from the fermentation broth of Trametes vers...
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Article
Gibberellic Acid Increases Secondary Metabolite Production in Echinacea purpurea Hairy Roots
Gibberellic acid (GA3) is reported to have diverse effects on hairy root cultures of many plant species; therefore, the effects of GA3 on the growth, secondary metabolite production (caffeic acid derivatives and ...
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Article
Heat Shock Treatment Improves Trametes versicolor Laccase Production
An efficient heat shock strategy has been developed to improve laccase production in submerged Trametes versicolor cultures. The optimized heat shock strategy consists of subjecting T. versicolor mycelial pellets...
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Article
Improved Mass Multiplication of Rhodiola crenulata Shoots Using Temporary Immersion Bioreactor with Forced Ventilation
A temporary immersion bioreactor system was found to be suitable for mass shoot proliferation of Rhodiola crenulata. The shoot multiplication ratio and hyperhydration rate reached 46.8 and 35.4%, respectively, at...