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Chapter
Neuron-Microglia Dynamic Duo in Chronic Abdominal Pain
Abdominal pain is one of the main symptoms of chronic gastrointestinal disorders including inflammatory bowel diseases (IBD) and irritable bowel syndrome (IBS). The pathological mechanisms of chronic visceral ...
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Article
TRPV1-Targeted Drugs in Development for Human Pain Conditions
The transient receptor potential vanilloid-1 (TRPV1) is a non-specific cation channel known for its sensitivity to pungent vanilloid compound (i.e. capsaicin) and noxious stimuli, including heat, low pH or inf...
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Article
Open AccessChronic morphine regulates TRPM8 channels via MOR-PKCβ signaling
Postoperative shivering and cold hypersensitivity are major side effects of acute and chronic opioid treatments respectively. TRPM8 is a cold and menthol-sensitive channel found in a subset of dorsal root gang...
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Article
Microbiota: a novel regulator of pain
Among the various regulators of the nervous system, the gut microbiota has been recently described to have the potential to modulate neuronal cells activation. While bacteria-derived products can induce aversi...
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Article
TRPV1 and MOR working in tandem: implications for pain and opioids use
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Article
Open AccessActivation of Peripheral Blood CD4+ T-Cells in IBS is not Associated with Gastrointestinal or Psychological Symptoms
Immune activation may underlie the pathogenesis of irritable bowel syndrome (IBS), but the evidence is conflicting. We examined whether peripheral CD4+ T-cells from IBS patients demonstrated immune activation ...
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Article
Open AccessItch induced by peripheral mu opioid receptors is dependent on TRPV1-expressing neurons and alleviated by channel activation
Opioids remain the gold standard for the treatment of moderate to severe pain. However, their analgesic properties come with important side effects, including pruritus, which occurs frequently after systemic o...
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Article
Open AccessProlonged AT1R activation induces CaV1.2 channel internalization in rat cardiomyocytes
The cardiac L-type calcium channel is a multi-subunit complex that requires co-assembling of the pore-forming subunit CaV1.2 with auxiliary subunits CaVα2δ and CaVβ. Its traffic has been shown to be controlled by...
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Article
CaV2.2 channel cell surface expression is regulated by the light chain 1 (LC1) of the microtubule-associated protein B (MAP1B) via UBE2L3-mediated ubiquitination and degradation
Microtubule-associated protein B is a cytoskeleton protein consisting of heavy and light (LC) chains that play important roles in the regulation of neuronal morphogenesis and function. LC1 is also well known t...
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Article
Sustained neurochemical plasticity in central terminals of mouse DRG neurons following colitis
Sensitization of dorsal root ganglia (DRG) neurons is an important mechanism underlying the expression of chronic abdominal pain caused by intestinal inflammation. Most studies have focused on changes in the p...
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Article
New neostigmine-based behavioral mouse model of abdominal pain
Animal models of visceral pain have gained much attention as an important tool to elucidate the possible mechanisms underlying functional gastrointestinal (GI) disorders. Here we report the development of a ne...
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Chapter
GPCR and Voltage-Gated Calcium Channels (VGCC) Signaling Complexes
Voltage-gated ion channels are transmembrane proteins that control nerve impulses and cell homeostasis. Signaling molecules that regulate ion channel activity and density at the plasma membrane must be specifi...
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Article
The Cavβ subunit prevents RFP2-mediated ubiquitination and proteasomal degradation of L-type channels
The auxiliary subunit Cavβ regulates calcium channel density in the plasma membrane, but the mechanism by which this occurs has been poorly defined. Altier et al. find that Cavβ prevents ubiquitination of the Cav...
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Article
PS IV -1
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Protocol
Analysis of GPCR/Ion Channel Interactions
Voltage-gated calcium channels are key regulators of calcium homeostasis in excitable cells. A number of cellular signaling pathways serve to fine tune calcium channel activity, including the activation of G p...
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Article
Regulation of T-type calcium channels by Rho-associated kinase
We investigated the regulation of T-type channels by Rho-associated kinase (ROCK). Activation of ROCK via the endogenous ligand lysophosphatidic acid (LPA) reversibly inhibited the peak current amplitudes of r...
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Article
Opioid, cheating on its receptors, exacerbates pain
Opioids are commonly thought of as compounds that alleviate pain. A new study finds that elevated levels of the opioid dynorphin can unexpectedly activate bradykinin receptors, contributing to the maintenance ...
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Article
ORL1 receptor–mediated internalization of N-type calcium channels
The inhibition of N-type calcium channels by opioid receptor like receptor 1 (ORL1) is a key mechanism for controlling the transmission of nociceptive signals. We recently reported that signaling complexes con...
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Article
Agonist-independent modulation of N-type calcium channels by ORL1 receptors
We have investigated modulation of voltage-gated calcium channels by nociceptin (ORL1) receptors. In rat DRG neurons and in tsA-201 cells, nociceptin mediated a pronounced inhibition of N-type calcium channels...
