TALENs
Methods and Protocols
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Protocol
Gene Editing in Mouse Zygotes Using the CRISPR/Cas9 System
Engineering of the mouse germline is a key technology in biomedical research for studying the function of genes in health and disease. Since the first knockout mouse was described in 1989, gene targeting was b...
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Article
Open AccessPhosphorylation of muramyl peptides by NAGK is required for NOD2 activation
Bacterial cell wall components provide various unique molecular structures that are detected by pattern recognition receptors (PRRs) of the innate immune system as non-self. Most bacterial species form a cell ...
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Article
Simple and reliable detection of CRISPR-induced on-target effects by qgPCR and SNP genoty**
The recent CRISPR revolution has provided researchers with powerful tools to perform genome editing in a variety of organisms. However, recent reports indicate widespread occurrence of unintended CRISPR-induce...
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Article
Open AccessThe Alzheimer’s disease-associated protective Plcγ2-P522R variant promotes immune functions
Microglia-specific genetic variants are enriched in several neurodegenerative diseases, including Alzheimer’s disease (AD), implicating a central role for alterations of the innate immune system in the disease...
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Open AccessCongenic expression of poly-GA but not poly-PR in mice triggers selective neuron loss and interferon responses found in C9orf72 ALS
Expansion of a (G4C2)n repeat in C9orf72 causes amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), but the link of the five repeat-encoded dipeptide repeat (DPR) proteins to neuroinflammation,...
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Open AccessThe Trem2 R47H Alzheimer’s risk variant impairs splicing and reduces Trem2 mRNA and protein in mice but not in humans
The R47H variant of the Triggering Receptor Expressed on Myeloid cells 2 (TREM2) significantly increases the risk for late onset Alzheimer’s disease. Mouse models accurately reproducing phenotypes observed in ...
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Article
Erratum: Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells
Nat. Biotechnol. 33, 543–548 (2015); published online 24 March 2015; corrected after print 14 December 2017 In the version of this article initially published, in the HTML only, in Online Methods “Cell culture...
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Control of gene editing by manipulation of DNA repair mechanisms
DNA double-strand breaks (DSBs) are produced intentionally by RNA-guided nucleases to achieve genome editing through DSB repair. These breaks are repaired by one of two main repair pathways, classic non-homolo...
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Protocol
Genome Editing in Mice Using TALE Nucleases
Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as sequence-specific nucleases to induce targeted double-strand breaks, the mouse genome can be ...
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Article
Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells
The efficiency of precise CRISPR/Cas9 genome editing is increased by inhibition of the nonhomologous end joining pathway.
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Article
Generation of targeted mouse mutants by embryo microinjection of TALEN mRNA
Genetically engineered mice are instrumental for the analysis of mammalian gene function in health and disease. As classical gene targeting, which is performed in embryonic stem (ES) cell cultures and generate...
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Article
Gezielte Manipulation des Genoms mit Zinkfingernukleasen
In vielen Säugetierarten gab es bis jetzt keine effektiven Werkzeuge für eine gezielte Genmodifikation. Die Entwicklung der Zinkfingernuklease-Technologie ermöglicht nun auch in diesen Organismen eine schnelle...
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Protocol
Generating Conditional Knockout Mice
Gene targeting in ES cells is extensively used to generate designed mouse mutants and to study gene function in vivo. Knockout mice that harbor a null allele in their germline provide appropriate genetic models o...
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Article
Local Knockdown of ERK2 in the Adult Mouse Brain Via Adeno-Associated Virus-Mediated RNA Interference
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Open AccessLocal Knockdown of ERK2 in the Adult Mouse Brain Via Adeno-Associated Virus-Mediated RNA Interference
In recent years RNA interference (RNAi) has become a useful genetic tool to downregulate candidate disease genes for which pharmaceutical inhibitors are not available. In combination with viral vectors to trig...
