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Article
Volatile apocarotenoid discovery and quantification in Arabidopsis thaliana: optimized sensitive analysis via HS-SPME-GC/MS
In the field of carotenoid metabolism researchers’ focus has been directed recently toward the discovery and quantification of carotenoid cleavage products (i.e. apocarotenoids, excluding the well-studied caro...
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Article
Convergence of mitochondrial and chloroplastic ANAC017/PAP-dependent retrograde signalling pathways and suppression of programmed cell death
The energy-converting organelles mitochondria and chloroplasts are tightly embedded in cellular metabolism and stress response. To appropriately control organelle function, extensive regulatory mechanisms are ...
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Article
Molecular characterization and transcriptome analysis of orange head Chinese cabbage (Brassica rapa L. ssp. pekinensis)
The orange head phenotype of Br - or resulted from a large insertion in carotenoid isomerase (BrCRTISO) . Comparative tra...
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Article
Open AccessGenomic breeding for food, environment and livelihoods
Land use management is a central challenge for the 21st century with unprecedented and competing demands to produce food, feed/fodder, fibre, fuel, and essential ecosystem services which sustain life. Global c...
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Chapter
An Overview of Chloroplast Biogenesis and Development
During evolution plants have incorporated chloroplasts into their cells. Within the chloroplast energy is produced during photosynthesis but also hormones and metabolites essential for plant metabolism. As a c...
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Protocol
Isolation of the Plant Cytosolic Fraction for Proteomic Analysis
The cytosol is the fluid portion of the cell that is not partitioned by membranes. It contains a highly diverse collection of substances and is central to many essential cellular processes ranging from signal ...
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Article
Open AccessInflorescence stem grafting made easy in Arabidopsis
Plant grafting techniques have deepened our understanding of the signals facilitating communication between the root and shoot, as well as between shoot and reproductive organs. Transmissible signalling molecu...
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Protocol
Identifying Chloroplast Biogenesis and Signalling Mutants in Arabidopsis thaliana
The chloroplast is the largest and arguably the most complex of the three energy organelles in the plant cell. The biogenesis of the chloroplast requires a combination of thousands of proteins encoded by the c...
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Article
Alternative splicing, activation of cryptic exons and amino acid substitutions in carotenoid biosynthetic genes are associated with lutein accumulation in wheat endosperm
Endosperm carotenoid content in wheat is a primary determinant of flour colour and this affects both the nutritional value of the grain and its utility for different applications. Utilising wheat rice synteny ...
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Article
Open AccessA rapid, non-invasive procedure for quantitative assessment of drought survival using chlorophyll fluorescence
Analysis of survival is commonly used as a means of comparing the performance of plant lines under drought. However, the assessment of plant water status during such studies typically involves detachment to es...
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Article
Quantification of cyclic electron flow around Photosystem I in spinach leaves during photosynthetic induction
The variation of the rate of cyclic electron transport around Photosystem I (PS I) during photosynthetic induction was investigated by illuminating dark-adapted spinach leaf discs with red + far-red actinic li...
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Chapter
Carotenoids
Carotenoid pigments provide fruit and flowers with distinctive red, orange and yellow colors and a number of aromas, which make them commercially important in agriculture, food manufacturing and the cosmetic i...
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Article
A simple chlorophyll fluorescence parameter that correlates with the rate coefficient of photoinactivation of Photosystem II
A method of partitioning the energy in a mixed population of active and photoinactivated Photosystem II (PS II) complexes based on chlorophyll fluorescence measurements is presented. There are four energy flux...
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Chapter
The Role of Carotenoids in Energy Quenching
Photoprotection remains one of the most challenging and complex areas for research in photosynthesis. Co** with a wide range of adverse environmental conditions, especially full sunlight, is central to plant...
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Article
Morphine-pathway block in top1 poppies
This opium poppy mutant provides chemical precursors for non-addictive analgesics.
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Protocol
Identifying Photoprotection Mutants in Arabidopsis thaliana
Plants have a range of mechanisms to protect against oxidative damage induced by excess light and environmental stress. One of these processes consists of the detoxification of reactive oxygen species by the a...
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Article
Occurrence of the lutein-epoxide cycle in mistletoes of the Loranthaceae and Viscaceae
The lutein-epoxide cycle (Lx cycle) is an auxiliary xanthophyll cycle known to operate only in some higher-plant species. It occurs in parallel with the common violaxanthin cycle (V cycle) and involves the sam...
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Article
Photoprotection in a zeaxanthin- and lutein-deficient double mutant of Arabidopsis
When light absorption by a plant exceeds its capacity for light utilization, photosynthetic light harvesting is rapidly downregulated by photoprotective thermal dissipation, which is measured as nonphotochemic...
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Article
Antisense inhibition of the beta-carotene hydroxylase enzyme in Arabidopsis and the implications for carotenoid accumulation, photoprotection and antenna assembly
The xanthophylls are oxygenated carotenoids and are important structural components of the photosynthetic apparatus. Xanthophylls contribute to the assembly and stability of light-harvesting complex apoprotein...
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Chapter
On the Respective Roles of the Various Xanthophylls During Seedling Development and High Light Stress in Arabidopsis
Six hundred different naturally occurring carotenoids have been identified to date and this diversity is reflected in the composition of the photosynthetic apparatus of different bacterial and algal classes. H...
