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Chapter
Targeted Sequencing in Coffee with the Daicel Arbor Biosciences Exome Capture Kit
Exome Capture is a molecular biology technique that, in combination with Next Generation DNA sequencing technologies (NGS), allows for selectively sequencing the predicted genes of an organism. Such capture se...
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Article
Open AccessAnalysis of DNAs associated with coconut foliar decay disease implicates a unique single-stranded DNA virus representing a new taxon
The unique ecology, pathology and undefined taxonomy of coconut foliar decay virus (CFDV), found associated with coconut foliar decay disease (CFD) in 1986, prompted analyses of old virus samples by modern met...
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Article
Open AccessGenomic breeding for food, environment and livelihoods
Land use management is a central challenge for the 21st century with unprecedented and competing demands to produce food, feed/fodder, fibre, fuel, and essential ecosystem services which sustain life. Global c...
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Article
Genome expansion of Arabis alpina linked with retrotransposition and reduced symmetric DNA methylation
Despite evolutionary conserved mechanisms to silence transposable element activity, there are drastic differences in the abundance of transposable elements even among closely related plant species. We conducte...
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Protocol
Artificial MicroRNAs for Specific Gene Silencing in Rice
Artificial microRNAs (amiRNAs) have been shown to facilitate efficient gene silencing with high specificity to the intended target gene(s). For the plant breeder, gene silencing by artificial miRNAs will certa...
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Protocol
Directed Gene Silencing with Artificial MicroRNAs
The characterization of gene function typically includes a detailed analysis of loss-of-function alleles. In model plants, such as Arabidopsis thaliana and rice, sequence-indexed insertion collections provide a l...
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Article
Open AccessSimultaneous alignment of short reads against multiple genomes
Genome resequencing with short reads generally relies on alignments against a single reference. GenomeMapper supports simultaneous map** of short reads against multiple genomes by integrating related genomes (e...
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Article
Open AccessESTs and EST-linked polymorphisms for genetic map** and phylogenetic reconstruction in the guppy, Poecilia reticulata
The guppy, Poecilia reticulata, is a well-known model organism for studying inheritance and variation of male ornamental traits as well as adaptation to different river habitats. However, genomic resources for st...
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Article
The PHYTOCHROME C photoreceptor gene mediates natural variation in flowering and growth responses of Arabidopsis thaliana
Light has an important role in modulating seedling growth and flowering time. We show that allelic variation at the PHYTOCHROME C (PHYC) photoreceptor locus affects both traits in natural populations of A. thalia...
