-
Article
The chromatin remodeller ACF acts as a dimeric motor to space nucleosomes
Evenly spaced nucleosomes directly correlate with condensed chromatin and gene silencing. The ATP-dependent chromatin assembly factor (ACF) forms such structures in vitro and is required for silencing in vivo. AC...
-
Article
Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila
Some viruses carry suppressors to inhibit host response to infection through the RNAi pathways. Previously the insect virus DCV was found to target host response by inhibiting Drosophila Dicer function. The relat...
-
Article
Mutational and fitness landscapes of an RNA virus revealed through population sequencing
A new approach to accurately determine mutation frequencies with RNA virus populations called circular sequencing (CirSeq) has been developed; a study of the genetic composition of populations of poliovirus sh...
-
Article
Library preparation for highly accurate population sequencing of RNA viruses
NGS error rates are very low in libraries prepared by CirSeq, allowing ultra-rare genetic variant detection in populations. Sequencing errors are easily identified as replicates of a particular sequence are ph...
-
Article
Open AccessHsp90 shapes protein and RNA evolution to balance trade-offs between protein stability and aggregation
Acquisition of mutations is central to evolution; however, the detrimental effects of most mutations on protein folding and stability limit protein evolvability. Molecular chaperones, which suppress aggregatio...
