103 Result(s)
-
Article
Noggin is a mesenchymally derived stimulator of hair-follicle induction
The induction of developmental structures derived from the ectoderm, such as the neural tube or tooth, occurs through neutralization of the inhibitory activity of members of the bone-morphogenetic protein (BMP...
-
Article
Sphingosylphosphorylcholine is a ligand for ovarian cancer G-protein-coupled receptor 1
Sphingosylphosphorylcholine (SPC) is a bioactive lipid that acts as an intracellular and extracellular signalling molecule in numerous biological processes. Many of the cellular actions of SPC are believed to ...
-
Article
PDGF-C is a new protease-activated ligand for the PDGF α-receptor
Platelet-derived growth factors (PDGFs) are important in many types of mesenchymal cell. Here we identify a new PDGF, PDGF-C, which binds to and activates the PDGF α-receptor. PDGF-C is activated by proteolysi...
-
Article
The cell biology of neuronal navigation
Morphogenesis of the nervous system requires the directed migration of postmitotic neurons to designated locations in the nervous system and the guidance of axon growth cones to their synaptic targets. Evidenc...
-
Article
Cytoplasmic localization of p21Cip1/WAF1 by Akt-induced phosphorylation in HER-2/neu-overexpressing cells
Amplification or overexpression of HER-2/neu in cancer cells confers resistance to apoptosis and promotes cell growth. The cellular localization of p21Cip1/WAF1 has been proposed to be critical either in promotin...
-
Article
Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly
SCF ubiquitin ligases are composed of Skp1, Cdc53, Hrt1 and one member of a large family of substrate receptors known as F-box proteins (FBPs). Here we report the identification, using sequential rounds of epi...
-
Article
SNX3 regulates endosomal function through its PX-domain-mediated interaction with PtdIns(3)P
The sorting nexin (SNX) protein family is implicated in regulating membrane traffic, but the mechanism is still unknown. We show that SNX3 is associated with the early endosome through a novel motif (PX domain...
-
Article
Dual role of the fringe connection gene in both heparan sulphate and fringe-dependent signalling events
The precise regulation of growth factor signalling is crucial to the molecular control of development in Drosophila. Post-translational modification of signalling molecules is one of the mechanisms that modulate ...
-
Article
Identification of an organelle receptor for myosin-Va
Little is known about how molecular motors bind to their vesicular cargo. Here we show that myosin-Va, an actin-based vesicle motor, binds to one of its cargoes, the melanosome, by interacting with a receptor–...
-
Article
Hid, Rpr and Grim negatively regulate DIAP1 levels through distinct mechanisms
Inhibitor of apoptosis (IAP) proteins suppress apoptosis and inhibit caspases. Several IAPs also function as ubiquitin-protein ligases. Regulators of IAP auto-ubiquitination, and thus IAP levels, have yet to b...
-
Article
Discs-Large and Strabismus are functionally linked to plasma membrane formation
During early embryogenesis in Drosophila melanogaster, extensive vesicle transport occurs to build cell boundaries for 6,000 nuclei. Here we show that this important process depends on a functional complex formed...
-
Article
The tumour suppressor RASSF1A regulates mitosis by inhibiting the APC–Cdc20 complex
The tumour suppressor gene RASSF1A is frequently silenced in lung cancer and other sporadic tumours as a result of hypermethylation of a CpG island in its promoter1,2,3,4,5,6. However, the precise mechanism by wh...
-
Article
Dynamin 2 binds γ-tubulin and participates in centrosome cohesion
Dynamin 2 (Dyn2) is a large GTPase involved in vesicle formation and actin reorganization1,2,3. In this study, we report a novel role for Dyn2 as a component of the centrosome that is involved in centrosome cohes...
-
Article
Guidance of myocardial patterning in cardiac development by Sema6D reverse signalling
Cardiac chamber formation involves dynamic changes in myocardial organization, including trabeculation and expansion of the compact layer1. The positional cues that regulate myocardial patterning, however, remain...
-
Article
Non-canonical Wnt signals are modulated by the Kaiso transcriptional repressor and p120-catenin
Gastrulation movements are critical for establishing the three principal germ layers and the basic architecture of vertebrate embryos. Although the individual molecules and pathways involved are not clearly un...
-
Article
Bcl-2 expression suppresses mismatch repair activity through inhibition of E2F transcriptional activity
Bcl-2 stimulates mutagenesis after the exposure of cells to DNA-damaging agents. However, the biological mechanisms of Bcl-2-mediated mutagenesis have remained largely obscure. Here we demonstrate that the Bcl...
-
Article
Actin and Arf1-dependent recruitment of a cortactin–dynamin complex to the Golgi regulates post-Golgi transport
Cortactin is an actin-binding protein that has recently been implicated in endocytosis. It binds directly to dynamin-2 (Dyn2), a large GTPase that mediates the formation of vesicles from the plasma membrane an...
-
Article
Ric-8 controls Drosophila neural progenitor asymmetric division by regulating heterotrimeric G proteins
Asymmetric division of Drosophila neuroblasts (NBs) and the Caenorhabditis elegans zygote uses polarity cues provided by the Par proteins, as well as heterotrimeric G-protein-signalling that is activated by a rec...
-
Article
Phosphatidylinositol transfer protein-α in netrin-1-induced PLC signalling and neurite outgrowth
Neurite extension is essential for wiring the nervous system during development. Although several factors are known to regulate neurite outgrowth, the underlying mechanisms remain unclear. Here, we provide evi...
-
Article
V-ATPase interacts with ARNO and Arf6 in early endosomes and regulates the protein degradative pathway
The recruitment of the small GTPase Arf6 and ARNO from cytosol to endosomal membranes is driven by V-ATPase-dependent intra-endosomal acidification. The molecular mechanism that mediates this pH-sensitive recr...
