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Article
Open AccessEnvironment modulates protein heterogeneity through transcriptional and translational stop codon readthrough
Stop codon readthrough events give rise to longer proteins, which may alter the protein’s function, thereby generating short-lasting phenotypic variability from a single gene. In order to systematically assess...
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Chapter and Conference Paper
Prospects for the Production of Recycled Hot Mix Asphalt with Plastic Fiber
One of the advanced research directions to reduce the cost of road construction is the production of hot recycled asphalt concrete mixes based on milled asphalt concrete, which is formed by cutting off layers ...
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Chapter and Conference Paper
Review of Engineering Research Methods for the Formation of a Digital Model of the Area with the Determination of the Accuracy and Compliance
The article considers the main methods of engineering surveys (leveling by diameters, tacheometric survey, laser survey, survey by laser profilers, GPS-survey) survey of the area with the main advantages and d...
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Article
Oral nutritional supplement prevents weight loss and reduces side effects in patients in advanced lung cancer chemotherapy
Weight loss in patients with cancer is caused by cancer cachexia and chemotherapy-induced nausea and vomiting. Recent developments in antiemetic drugs have substantially improved nausea and vomiting, but this ...
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Chapter and Conference Paper
Analysis of Existing Train Lines and International Railway Transport Corridors of Ukraine
In this paper, the characteristics of international transport corridors (ITC) with common technical parameters and ensuring the application of a unified transportation technology as the basis for the integrati...
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Article
Open AccessChromatin Central: towards the comparative proteome by accurate map** of the yeast proteomic environment
Understanding the design logic of living systems requires the understanding and comparison of proteomes. Proteomes define the commonalities between organisms more precisely than genomic sequences. Because unce...
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Article
Open AccessOntogenetic variations in the venom proteome of the Amazonian snake Bothrops atrox
Bothrops atrox is responsible for the majority of snakebite accidents in the Brazilian Amazon region. Previous studies have demonstrated that the biological and pharmacological activities of B. atrox venom alter ...
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Article
Open AccessIdentification of glycosylated marker proteins of epithelial polarity in MDCK cells by homology driven proteomics
MDCK cells derived from canine kidney are an important experimental model system for investigating epithelial polarity in mammalian cells. Monoclonal antibodies against apical gp114 and basolateral p58 have se...
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Article
Excipient selection can significantly affect solid-state phase transformation in formulation during wet granulation
Phase transformations in formulations can lead to instability in physicochemical, biopharmaceutical, and processing properties of products. The influences of formulation design on the optimal dosage forms shou...
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Protocol
Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes
Recent developments in technology and instrumentation have made mass spectrometry the method of choice for identification of proteins in a sequence database [reviewed in refs. (1–4)].Regardless of what mass spect...
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Article
“De novo” sequencing of peptides recovered from in-gel digested proteins by nanoelectrospray tandem mass spectrometry
Proteins separated by one-dimensional or two-dimensional gel electrophoresis can be digested in-gel with trypsin and the recovered peptides can be sequenced de novo using triple quadrupole or hybrid quadrupole ti...
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Article
Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly
SCF ubiquitin ligases are composed of Skp1, Cdc53, Hrt1 and one member of a large family of substrate receptors known as F-box proteins (FBPs). Here we report the identification, using sequential rounds of epi...
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Article
A generic protein purification method for protein complex characterization and proteome exploration
We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification...
