22 Result(s)
within
Stephen M. Beverley
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Article
Open AccessDiversity and dissemination of viruses in pathogenic protozoa
Viruses are the most abundant biological entities on Earth and play a significant role in the evolution of many organisms and ecosystems. In pathogenic protozoa, the presence of viruses has been linked to an i...
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Article
Leishmania genetic exchange is mediated by IgM natural antibodies
Host factors that mediate Leishmania genetic exchange are not well defined. Here we demonstrate that natural IgM (IgMn)1–4 antibodies mediate parasite genetic exchange by inducing the transient formation of a sph...
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Article
The Evolutionary Loss of RNAi Key Determinants in Kinetoplastids as a Multiple Sporadic Phenomenon
We screened the genomes of a broad panel of kinetoplastid protists for genes encoding proteins associated with the RNA interference (RNAi) system using probes from the Argonaute (AGO1), Dicer1 (DCL1), and Dice...
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Article
Open AccessComparative genomic analysis of Leishmania (Viannia) peruviana and Leishmania (Viannia) braziliensis
The Leishmania (Viannia) braziliensis complex is responsible for most cases of New World tegumentary leishmaniasis. This complex includes two closely related species but with different geographic distribution and...
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Article
CRISPR for Cryptosporidium
Study of the diarrhoea-causing pathogen Cryptosporidium has been hindered by a lack of genetic-modification and culture tools. A description of genome editing and propagation methods for the parasite changes this...
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Article
Open AccessREDHORSE-REcombination and Double crossover detection in Haploid Organisms using next-geneRation SEquencing data
Next-generation sequencing technology provides a means to study genetic exchange at a higher resolution than was possible using earlier technologies. However, this improvement presents challenges as the alignm...
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Protocol
A Transposon-Based Tool for Transformation and Mutagenesis in Trypanosomatid Protozoa
The ability of transposable elements to mobilize across genomes and affect the expression of genes makes them exceptional tools for genetic manipulation methodologies. Several transposon-based systems have bee...
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Article
A transposon toolkit for gene transfer and mutagenesis in protozoan parasites
Protozoan parasites affect millions of people around the world. Treatment and control of these diseases are complicated partly due to the intricate biology of these organisms. The interactions of species of Plasm...
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Chapter
Sphingolipids in Parasitic Protozoa
The surface of most protozoan parasites relies heavily upon lipid-anchored molecules, to form protective barriers and play critical functions required for infectivity. Sphingolipids (SLs) play impo...
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Article
PTR1-dependent synthesis of tetrahydrobiopterin contributes to oxidant susceptibility in the trypanosomatid protozoan parasite Leishmania major
Leishmania must survive oxidative stress, but lack many classical antioxidant enzymes and rely heavily on trypanothione-dependent pathways. We used forward genetic screens to recover loci...
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Article
Characterization of inositol phosphorylceramides from Leishmania major by tandem mass spectrometry with electrospray ionization
We describe tandem mass spectrometric approaches, including multiple stage ion-trap and source collisionally activated dissociation (CAD) tandem mass spectrometry with electrospray ionization (ESI) to characte...
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Article
Central memory T cells mediate long-term immunity to Leishmania major in the absence of persistent parasites
Infection with Leishmania major induces a protective immune response and long-term resistance to reinfection, which is thought to depend upon persistent parasites. Here we demonstrate that although effector CD4+ ...
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Protocol
In Vitro Shuttle Mutagenesis Using Engineered Mariner Transposons
Advances in our understanding of the protozoan parasite Leishmania have been facilitated by the development of molecular and genetic tools. One powerful approach for gene identification and analysis is transposon...
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Article
Protozomics: trypanosomatid parasite genetics comes of age
Trypanosomatid protozoans are responsible for some of the most devastating and, as yet, poorly controlled diseases of tropical and semi-tropical countries.
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Article
The Leishmania genome project: new insights into gene organization and function
The sequencing of Leishmania major Friedlin chromosome 1 (Chr1), Chr3, and Chr4 has been completed, and several other chromosomes are well underway. The complete genome sequence should be available by 2003. Over...
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Article
Dual role of the fringe connection gene in both heparan sulphate and fringe-dependent signalling events
The precise regulation of growth factor signalling is crucial to the molecular control of development in Drosophila. Post-translational modification of signalling molecules is one of the mechanisms that modulate ...
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Article
Pteridine reductase mechanism correlates pterin metabolism with drug resistance in trypanosomatid parasites
Pteridine reductase (PTR1) is a short-chain reductase (SDR) responsible for the salvage of pterins in parasitic trypanosomatids. PTR1 catalyzes the NADPH-dependent two-step reduction of oxidized pterins to the...
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Protocol
Transfection ofLeishmania and Trypanosoma brucei by Electroporation
DNA transfection is the introduction of molecular constructs into parasites in a manner permitting the expression of encoded genes. Transfection is an exceedingly powerful technique for analyzing gen etic regu...
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Article
Gene replacement in parasitic protozoa
TRYPANOSOMATID protozoa frequently cause severe diseases in humans. Many molecules likely to have a role during the infectious cycle have been identified, yet proof of their function is often lacking. We descr...
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Chapter
Unstable and Stable Gene Amplification in Methotrexate-Resistant Leishmania Major and Natural Isolates of Leishmania Tarentolae
One mechanism that cells and organisms can employ to increase the level of gene expression is that of specific gene amplification. This process has been most intensively studied in tumor cells exhibiting ampli...
