Search
Search Results
-
A Brief Practical Guide to PCR
Polymerase chain reaction (PCR) has been a powerful molecular biology tool since the mid-1980s. Millions of copies of specific sequence regions of... -
Nuclease Enrichment and qPCR Detection of Rare Nucleotide Variants
The emergence of circulating DNA analysis in blood during the past decade has responded to the need for noninvasive alternatives to classical tissue... -
Ultrasensitive Reverse Transcriptase Loop-Mediated Isothermal Amplification (US-LAMP)-Based Detection of Malaria Infection from Dried Blood Spots
Submicroscopic malaria diagnosis requires highly sensitive tools instead of the conventional microscopy and rapid diagnostic tests (RDTs). While... -
-
Measuring Enzyme Kinetics of Glycoside Hydrolases Using the 3,5-Dinitrosalicylic Acid Assay
Use of the 3,5-dinitrosalicylic acid reagent allows the simple, rapid quantification of reducing sugars. The method can be used for analysis of... -
CBMs as Probes to Explore Plant Cell Wall Heterogeneity Using Immunocytochemistry
Immunocytochemistry is a widely used technique to localize antigen within intact tissues. Plant cell walls are complex matrixes of highly decorated... -
Site-Specific Incorporation of Sulfotyrosine into Proteins in Mammalian Cells
Protein tyrosine O-sulfationposttranslational modifications (PTMs)tyrosine O-sulfation (PTS) plays a crucial role in numerous extracellular... -
Genetic Encoding of a Fluorescent Noncanonical Amino Acid as a FRET Donor for the Analysis of Deubiquitinase Activities
The genetic code expansion technology enables the genetic encoding of fluorescent noncanonical amino acids (ncAAs) for site-specific fluorescent... -
SARS-CoV-2 Sequencing for Variant Surveillance
In this chapter, next-generation sequencing of the entire viral genome of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is described.... -
Loop-Mediated Isothermal Amplification on Paper Microfluidic Chips for Highly Sensitive and Specific Zika Virus Detection Using Smartphone
Zika virus (ZIKV) infection may cause serious birth defects and is a critical concern for women of child-bearing age in affected regions. A simple,... -
Affinity Electrophoresis for Analysis of Catalytic Module–Carbohydrate Interactions
Affinity electrophoresis has long been used to study the interaction between proteins and large soluble ligands. The technique has been found to have... -
A Rapid Protocol for Preparing 8-Aminopyrene-1,3,6-Trisulfonate-Labeled Glycans for Capillary Electrophoresis-Based Enzyme Assays
Purified glycan standards are required for glycan arrays, characterizing substrate specificities of glycan-active enzymes, and to serve as... -
Qualitative and Quantitative Characterization of Protein–Carbohydrate Interactions by NMR Spectroscopy
Solution-state nuclear magnetic resonance (NMR) spectroscopy can be used to monitor protein–carbohydrate interactions. Two-dimensional 1H-15N... -
Next-Generation Sequencing Informatic Architecture Considerations
Clinically relevant sequencing methodologies continue to expand in number, diversity, complexity, and scale. This evolving and varied landscape... -
Tuberculosis Diagnosis Using Isothermal Nucleic Acid Amplification in a Paper-and-Plastic Device
Nucleic acid (DNA/RNA) amplification technologies are indispensable for applications like disease diagnostics, forensics, epidemiology, evolutionary... -
Immune Cell Lineage-Specific Chimerism Testing by Next-Generation Sequencing for Engraftment Monitoring After Allogeneic Hematopoietic Stem Cell Transplantation
Chimerism is an unusual state in which a person’s body comprises cells from genetically different people. Chimerism testing allows monitoring for the... -
Quantifying CBM–Carbohydrate Interactions Using Microscale Thermophoresis
Microscale thermophoresis (MST) is an emerging technology for studying a broad range of biomolecular interactions with a high sensitivity. The... -
Genetically Encoded Noncanonical Amino Acids in Proteins to Investigate Lysine Benzoylation
Posttranslational modifications (PTMs) of lysine residues are major regulators of gene expression, protein–protein interactions, and protein... -
Focused Engineering of Pyrrolysyl-tRNA Synthetase-Based Orthogonal Translation Systems for the Incorporation of Various Noncanonical Amino Acids
The expansion of the genetic code has become a valuable tool for molecular biology, biochemistry, and biotechnology. The pyrrolysyl-tRNA synthetase... -
Encoding Noncanonical Amino Acids into Phage Displayed Proteins
Phage display facilitates the evolution of peptides and proteins for affinity selection against targets, but it is mostly limited to the chemical...