Abstract
Microscale thermophoresis (MST) is an emerging technology for studying a broad range of biomolecular interactions with a high sensitivity. The affinity constant can be obtained for a wide range of molecules within minutes based on reactions in microliters. Here we describe the application of MST in quantifying protein–carbohydrate interactions. A CBM3a and a CBM4 are titrated with insoluble substrate (cellulose nanocrystal) and soluble oligosaccharide (xylohexaose), respectively.
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Acknowledgment
We acknowledge the Fédération de Recherche Agrobiosciences, Interactions et Biodiversité (FR 3450), CNRS, Université de Toulouse, UPS, Castanet-Tolosan, France, and the IDEX “UNITI” Université de Toulouse (GO-AHEAD project) for the NanoTemper Monolith NT.115 facilities.
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Wu, H., Montanier, C.Y., Dumon, C. (2023). Quantifying CBM–Carbohydrate Interactions Using Microscale Thermophoresis. In: Abbott, D.W., Zandberg, W.F. (eds) Carbohydrate-Protein Interactions. Methods in Molecular Biology, vol 2657. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3151-5_7
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DOI: https://doi.org/10.1007/978-1-0716-3151-5_7
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