-
Protocol
Characterization of Thermoresponsive Photobody Dynamics
Photobodies (PBs) are subnuclear membraneless organelles that self-assemble via the condensation of the plant photoreceptor and thermosensor phytochrome B (phyB). Changes in the light and temperature environme...
-
Protocol
Enzymatic Preparation of DNA with an Expanded Genetic Alphabet Using Terminal Deoxynucleotidyl Transferase and Its Applications
Efficient preparation of DNA oligonucleotides containing unnatural nucleobases (UBs) that can pair with their cognates to form unnatural base pairs (UBPs) is an essential prerequisite for the application of UB...
-
Protocol
A Nonviral piggyBac Transposon-Mediated Method to Generate Large-Scale CAR-NK Cells from Human Peripheral Blood Primary NK Cells
With the inherent antitumor function and unique “off-the-shelf” potential, genetically engineered human natural killer (NK) cells with chimeric antigen receptors (CARs) bear great promise for the treatment of ...
-
Protocol
A Magnetic Pincher for the Dynamic Measurement of the Actin Cortex Thickness in Live Cells
The actin cortex is an essential element of the cytoskeleton allowing cells to control and modify their shape. It is involved in cell division and migration. However, probing precisely the physical properties ...
-
Protocol
RNA Secondary Structure Modeling Following the IPANEMAP Workflow
The structure of RNA molecules and their complexes are crucial for understanding biology at the molecular level. Resolving these structures holds the key to understanding their manifold structure-mediated func...
-
Protocol
Ethanol Injection Method for Liposome Preparation
Ethanol injection method is one of the preferred methods for liposome preparation due to its advantages including rapidity, safety, and reproducibility. This method involves the injection of phospholipid solut...
-
Protocol
Metabolite Extraction from RPE Cells and Retinas Related to Retinitis Pigmentosa
The application of metabolomics in ophthalmology helps to identify new biomarkers and elucidate disease mechanisms in different eye diseases, as well as aiding in the development of potential treatment options...
-
Protocol
Plant Nuclei Isolation for Single-Nucleus RNA Sequencing
Transcriptome profiling has been significantly hampered by the heterogeneity among individual cells within a tissue or an organ. Recent advances in single cell transcriptome profiling have significantly advanc...
-
Protocol
Tracking Gut Motility in Organ and Cultures
Gastrointestinal (GI) motility is a key component of digestive health, and it is complex, involving a multitude of cell types and mechanisms to drive both rhythmic and arrhythmic activity. Tracking GI motility...
-
Protocol
Mass Spectrometry-Based Methods to Determine the Substrate Specificities and Kinetics of N-Linked Glycan Hydrolysis by Endo-β-N-Acetylglucosaminidases
Glycosylation is a common posttranslational modification of proteins and refers to the covalent addition of glycans, chains of polysaccharides, onto proteins producing glycoproteins. The glycans influence the ...
-
Protocol
Large-Scale Expansion of Umbilical Cord Mesenchymal Stem Cells with Microcarrier Tablets in Bioreactor
Mesenchymal stem cells show great potential in tissue engineering and cell-based therapies. This protocol demonstrates the use of 3D TableTrix® microcarrier tablets for large-scale manufacturing of human umbilica...
-
Protocol
DNA-Compatible Nitro Reduction and Synthesis of Benzimidazoles
A key factor for productive DNA-encoded libraries is the chemical diversity of the small molecule moiety attached to an encoding DNA oligomer. The library structure diversity is often limited to DNA-compatible...
-
Protocol
Bile Duct-on-a-Chip
Cholangiopathies affect the biliary tree via various pathophysiological mechanisms. Research on biliary physiology and pathology, however, is hampered by a lack of physiologically relevant in vitro models. Con...
-
Protocol
Measuring Autophagosome Flux
Autophagy is an evolutionarily conserved catabolic process that plays an import role in cellular proteostasis. The continual degradation, and recycling, of portions of the cytoplasm through autophagy eliminate...
-
Protocol
Construction of Strong Promoters by Assembling Sigma Factor Binding Motifs
Development of strong promoters is of growing interest in the field of biotechnology and synthetic biology. Here we present a protocol for the construction of strong prokaryotic promoters that can be recognize...
-
Protocol
Production of Virus-Free Chrysanthemum (Chrysanthemum morifolium Ramat) by Tissue Culture Techniques
Almost all plants in their natural environment are commonly infected by viruses. These viral infections can cause devastating diseases and result in severe yield and economic losses, making viral diseases an i...
-
Protocol
Gene Co-expression Network Analysis and Linking Modules to Phenoty** Response in Plants
Environmental factors, including different stresses, can have an impact on the expression of genes and subsequently the phenotype and development of plants. Since a large number of genes are involved in respon...
-
Protocol
Measurement of VEGF Content in Exosomes and Subsequent Tumor Tubulogenesis and In Vivo Angiogenesis Functional Assays
Vascular endothelial growth factor (VEGF) plays a vital role in angiogenesis, and is also involved in tumor cell growth and immunosuppression, showing very complex roles. VEGF-exosomes are released by tumor en...
-
Protocol
Quantification of Receptor Association, Dissociation, and NA-Dependent Motility of Influenza A Particles by Biolayer Interferometry
We describe a method for real-time analysis and quantification of influenza A virus (IAV)–receptor interactions by biolayer interferometry (BLI). Biotinylated synthetic sialoglycans or sialoglycoproteins (biot...
-
Protocol
Guidance for SARS-CoV-2 RNA-Based Molecular Assay Analytical Performance Evaluations
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is frequently diagnosed through detection of viral RNA using nucleic acid amplification testing (NAAT) assays that are usually used in cen...
