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Thermomorphogenesis

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Meng Chen

    1. Department of Botany and Plant Sciences, Institute for Integrative Genome Biology, University of California, Riverside, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2795)

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About this book

This detailed collection explores thermomorphogenesis, a discipline that unravels the intricacies of warm temperature responses in plants. How plants perceive, interpret, and respond to elevated temperatures has far-reaching implications for agriculture, ecology, and our fundamental understanding of plant development, and this book presents techniques to help researchers delve into this area of study. Beginning with a section on thermomorphogenesis responses, the volume continues with numerous chapters on temperature sensing and temperature signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Thermomorphogenesis: Methods and Protocols serves as an ideal guide to this vital subject for research laboratories, as well as for graduate and undergraduate laboratory courses in plant molecular biology.

Keywords

Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Thermomorphogenesis Responses

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. A Guide to Quantify Arabidopsis Seedling Thermomorphogenesis at Single Timepoints and by Interval Monitoring

      • Philipp Janitza, Zihao Zhu, Muhammad Usman Anwer, Martijn van Zanten, Carolin Delker
      Pages 3-16

    3. Dissection of Daytime and Nighttime Thermoresponsive Hypocotyl Elongation in Arabidopsis

      • De Fan, Meng Chen
      Pages 17-23

    4. Light-Dependent High Ambient Temperature-Induced Senescence Assay Using Whole Seedlings

      • Chanhee Kim, Giltsu Choi
      Pages 25-35

    5. Assessing Temperature Responses in Roots

      • Sanghwa Lee, Wolfgang Busch
      Pages 37-42

    6. A Luciferase Imaging-Based Assay for Studying Temperature Compensation of the Circadian Clock

      • Hongtao Zhang, Stacey L. Harmer
      Pages 43-53

    7. Investigating the Effects of Temperature on Pathogen Propagation in Arabidopsis

      • Zhan Li, Yang Liu, Jian Hua
      Pages 55-64

    8. Assessing the High Temperature Effects on Stomatal Production

      • Rini Rahiman, On Sun Lau
      Pages 65-73

    9. Characterizing the Synergistic Response Induced by Warm Temperatures and Shade

      • Yogev Burko
      Pages 75-81

  3. Temperature Sensing in Thermomorphogenesis

    1. Front Matter

      Pages 83-83
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    2. Measurement of Phytochrome B Thermal Reversion Rates In Vivo

      • Cornelia Klose, Andreas Hiltbrunner
      Pages 85-93

    3. Characterization of Thermoresponsive Photobody Dynamics

      • Juan Du, Meng Chen
      Pages 95-104

    4. Phase Separation of Phytochrome B in HEK293T Cells

      • Di Chen, Shangwei Zhong
      Pages 105-111

    5. Isolation of Phytochrome B Photobodies

      • Yongmin Kwon, Chanhee Kim, Giltsu Choi
      Pages 113-122

    6. Analysis of Phase Separation of EARLY FLOWERING 3

      • Maolin Peng, Stephanie Hutin, Aleksandra Mironova, Chloe Zubieta, Philip A. Wigge
      Pages 123-134

    7. In Vitro Determination of Temperature-Dependent DNA Binding of the Evening Complex Using Electrophoretic Mobility Shift Assays

      • Stephanie Hutin, Philip A. Wigge, Chloe Zubieta
      Pages 135-147

    8. Monitoring Real-Time Temperature Dynamics of a Short RNA Hairpin Using Förster Resonance Energy Transfer and Circular Dichroism

      • Martin Balcerowicz, Philip A. Wigge, Marco Di Antonio, Betty Chung
      Pages 149-158

  4. Temperature Signaling in Thermomorphogenesis

    1. Front Matter

      Pages 159-159
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    2. Characterization of PIF4 Phosphorylation by SPA1

      • Sanghwa Lee, Enamul Huq
      Pages 161-167
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Editors and Affiliations

  • Department of Botany and Plant Sciences, Institute for Integrative Genome Biology, University of California, Riverside, USA

    Meng Chen

Bibliographic Information

  • Book Title: Thermomorphogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Meng Chen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3814-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3813-2Published: 10 April 2024

  • Softcover ISBN: 978-1-0716-3816-3Due: 17 May 2024

  • eBook ISBN: 978-1-0716-3814-9Published: 17 May 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 264

  • Number of Illustrations: 7 b/w illustrations, 53 illustrations in colour

  • Topics: Plant Sciences

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