1,342 Result(s)
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Protocol
Ke** Zhang
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Protocol
Generating Neuroimmune Assembloids Using Human Induced Pluripotent Stem Cell (iPSC)-Derived Cortical Organoids and Microglia
The emergence of brain organoids has revolutionized our understanding of neurodevelopment and neurological diseases by providing an in vitro model system that recapitulates key aspects of human brain developme...
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TCID50 Assay: A Simple Method to Determine Baculovirus Titer
There are many methods that can be used to determine the infectious titer of your baculovirus stock. The TCID50 method is a simple end-point dilution method that determines the amount of baculovirus virus needed ...
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Production of Secreted Antibody in Baculovirus Expression Vector System
Monoclonal antibodies have widespread applications in disease treatment and antigen detection. They are traditionally produced using mammalian cell expression system, which is not able to satisfy the increasin...
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Assessment of Protein–Protein Docking Models Using Deep Learning
Protein–protein interactions are involved in almost all processes in a living cell and determine the biological functions of proteins. To obtain mechanistic understandings of protein–protein interactions, the ...
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Protocol
The Photothrombotic Model of Ischemic Stroke
Stroke is a major cause of morbidity worldwide; yet, there is a lack of treatment options to address post-stroke cognitive and motor impairment, thus there is an urgency for develo** neuroprotective and rest...
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Encoding Genetic Circuits with DNA Barcodes Paves the Way for High-Throughput Profiling of Dose-Response Curves of Metabolite Biosensors
Metabolite biosensors, through which the intracellular metabolite concentrations could be converted to changes in gene expression, are widely used in a variety of applications according to the different output...
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Split-Luciferase Complementation Imaging Assay in Virus–Plant Interactions
Protein−protein interactions constitute the interface between a virus and the cell it infects and are crucial determinants of the outcome of the viral infection. Multiple techniques have been developed to stud...
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Mass Spectrometry in Clinical Laboratory: Applications in Therapeutic Drug Monitoring and Toxicology
Mass spectrometry (MS) coupled with liquid chromatography (LC) or gas chromatography (GC) has been proven to be a powerful platform in research and specialized clinical laboratories for decades. In clinical la...
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Structural Determination of Glucosyltransferase C by Cryo-Electron Microscopy
Biofilm formation is a critical factor in the development of cariogenic virulence of Streptococcus mutans. S. mutans has evolved a concerted mechanism to synthesize a biofilm matrix from dietary sugars by a famil...
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Transiently Induce RNA Silencing in Plants Using a Tobacco Necrosis Virus A (TNV-A)-Based dsRNA Production System
Transgenic expression of hairpin RNA or artificial microRNA is widely used for genetic studies in plant science. However, induction of RNA silencing by transgenic method may have a problem when studying essent...
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The Direct Symbiotic Germination of Orchid Seeds in the Field
Mycorrhizal fungi are essential to the sustainability of the orchid population. Due to the minute nature of seeds, the mycobiont that the seedling can parasitize is considered an important element. Mycobionts ...
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Detection of dsRNA with Fluorescence In Situ Hybridization (FISH)
Fluorescence in situ hybridization (FISH) is a powerful technique used for detecting and localizing specific nucleic acid sequences in cells or tissues. Double-stranded RNA (dsRNA) is a type of RNA with comple...
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Induction of Meiotic Initiation in Long-Term Mouse Spermatogonial Stem Cells Under Retinoid Acid and Nutrient Restriction Conditions
Spermatogonial stem cells (SSCs) produce haploid sperm via mitosis and meiosis in vivo. Although the technique to culture mouse SSCs has been well established, induction of meiosis in vitro has remained a chal...
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Determination of De Novo Suberin-Lignin Ferulate Deposition in Xylem Tissue Upon Vascular Pathogen Attack
Plant vascular pathogens use different ways to reach the xylem vessels and cause devastating diseases in plants. Resistant and tolerant plants have evolved various defense mechanisms against vascular pathogens...
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High-Throughput Quantitative LC-MS/MS Analysis of Barbiturates in Human Urine
We hereby present a fast, high-throughput, and clinical LC-MS/MS assay for the simultaneous analysis of barbiturates in human urine. It is deployed as a quantitative assay for phenobarbital, butalbital, pentob...
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Super-Resolution Microscopy of the Bacterial Cell Wall Labeled by Fluorescent D-Amino Acids
Fluorescent D-amino acids (FDAAs) enable in situ visualization of bacterial cell wall synthesis via their incorporation into peptidoglycan (PG) crosslinks. When combined with super-resolution microscopy, FDAAs...
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Generation of Human Induced Pluripotent Stem Cells Using Episomal Vectors
Human induced pluripotent stem cells (iPSCs) can be reprogrammed from differentiated somatic cells, such as adult fibroblasts and peripheral blood mononuclear cells, by introducing sets of defined factors. iPS...
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In Silico: Predicting Intrinsic Features of HLA Class-I Restricted Neoantigens
Mutation-containing immunogenic peptides from tumor cells, also named as neoantigens, have various amino acid descriptors and physical-chemical properties characterized intrinsic features, which are useful in ...
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Quantitation of Urine Nicotine, Cotinine, and 3-OH-Cotinine by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Nicotine is a naturally occurring and highly addictive chemical used in e-cigarettes, cigarettes, chewing tobacco, and other tobacco products as well as in nicotine replacement therapies. The negative health c...
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Profiling Numerical and Structural Chromosomal Instability in Different Cancer Types
Many cancers display whole chromosome instability (W-CIN) and structural chromosomal instability (S-CIN), referring to increased rates of acquiring numerically and structurally abnormal chromosome changes. Thi...
