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Article
Resolution in super-resolution microscopy — definition, trade-offs and perspectives
Super-resolution microscopy (SRM) is gaining popularity in biosciences; however, claims about optical resolution are contested and often misleading. In this Viewpoint, experts share their views on resolution a...
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Article
Live-cell imaging powered by computation
The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. The prevailing challenge in live-cell fluorescence microscopy is c...
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Article
Open AccessChromosome organization shapes replisome dynamics in Caulobacter crescentus
DNA replication in bacteria takes place on highly compacted chromosomes, where segregation, transcription, and repair must occur simultaneously. Within this dynamic environment, colocalization of sister replis...
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Protocol
Super-Resolution Microscopy of the Bacterial Cell Wall Labeled by Fluorescent D-Amino Acids
Fluorescent D-amino acids (FDAAs) enable in situ visualization of bacterial cell wall synthesis via their incorporation into peptidoglycan (PG) crosslinks. When combined with super-resolution microscopy, FDAAs...
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Article
Optical microscopy gets down to angstroms
Super-resolution microscopy at ångström precision could pave the way to optical structural biology in cells.
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Article
Event-driven acquisition for content-enriched microscopy
A common goal of fluorescence microscopy is to collect data on specific biological events. Yet, the event-specific content that can be collected from a sample is limited, especially for rare or stochastic proc...
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Article
Author Correction: Single-molecule localization microscopy
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Article
Imaging cell biology
Imaging technologies drive discovery in cell biology. Innovations in microscopy hardware, imaging methods and computational analysis of large-scale, complex datasets can increase imaging resolution, definition...
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Article
Single-molecule localization microscopy
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can im...
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Article
Distinct fission signatures predict mitochondrial degradation or biogenesis
Mitochondrial fission is a highly regulated process that, when disrupted, can alter metabolism, proliferation and apoptosis1–3. Dysregulation has been linked to neurodegeneration3,4, cardiovascular disease3 and c...
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Article
Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics
Mitochondria contain the genetic information and expression machinery to produce essential respiratory chain proteins. Within the mitochondrial matrix, newly synthesized RNA, RNA processing proteins and mitori...
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Article
Homogeneous multifocal excitation for high-throughput super-resolution imaging
Super-resolution microscopies have become an established tool in biological research. However, imaging throughput remains a main bottleneck in acquiring large datasets required for quantitative biology. Here w...
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Article
Open AccessWaveguide-PAINT offers an open platform for large field-of-view super-resolution imaging
Super-resolution microscopies based on the localization of single molecules have been widely adopted due to their demonstrated performance and their accessibility resulting from open software and simple hardwa...
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Article
EZH2 oncogenic mutations drive epigenetic, transcriptional, and structural changes within chromatin domains
Chromatin is organized into topologically associating domains (TADs) enriched in distinct histone marks. In cancer, gain-of-function mutations in the gene encoding the enhancer of zeste homolog 2 protein (EZH2...
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Article
Multicolor single-particle reconstruction of protein complexes
Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a c...
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Article
TORC1 organized in inhibited domains (TOROIDs) regulate TORC1 activity
In budding yeast, glucose withdrawal, via the Rag GTPases, leads to TORC1 inhibition through its re-organization into a giant, vacuole-associated helix named a TOROID (TORC1 organized in inhibited domain).
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Article
Super-resolution imaging of multiple cells by optimized flat-field epi-illumination
An epi-illumination system based on microlens arrays enables field-independent imaging of multiple cells with nanoscale resolution and large field of views.
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Article
Open AccessIn Situ Characterization of Bak Clusters Responsible for Cell Death Using Single Molecule Localization Microscopy
Apoptosis plays a pivotal role in development and tissue homeostasis in multicellular organisms. Clustering of Bak proteins on the mitochondrial outer membrane is responsible for the induction of apoptosis by ...
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Article
Quantitative evaluation of software packages for single-molecule localization microscopy
This Analysis reports a comparison of current software packages for single-molecule localization in localization-based super-resolution imaging. Performance of the participating software on synthetic, biologic...
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Article
Open AccessFALCON: fast and unbiased reconstruction of high-density super-resolution microscopy data
Super resolution microscopy such as STORM and (F)PALM is now a well known method for biological studies at the nanometer scale. However, conventional imaging schemes based on sparse activation of photo-switcha...
