61 Result(s)

Article

Ribozyme-mediated repair of defective mRNA by targeted trans-splicing

RIBOZYMES can be targeted to cleave specific RNAs^1–8, which has led to much interest in their potential as gene inhibitors^3,9,10. Such fra/is-cleaving ribozymes join a growing list of agents that stop the flow of...

Bruce A. Sullenger, Thomas R. Cech in Nature (1994)

Article

Representation of the secondary and tertiary structure of group I introns

Group I introns, which are widespread in nature, carry out RNA self–splicing. The secondary structure common to these introns was for the most part established a decade ago. Information about their higher orde...

Thomas R. Cech, Simon H. Damberger, Robin R. Gutell in Nature Structural Biology (1994)

Article

Translocation of an RNA duplex on a ribozyme

RNA cleavage by the Tetrahymena ribozyme requires recognition of the reaction–site helix by the catalytic apparatus. This binding can occur in several registers, each of which results in reaction at a different n...

Scott A. Strobel, Thomas R. Cech in Nature Structural Biology (1994)

Article

Fishing for fresh catalysts

Thomas R. Cech in Nature (1993)

Article

Metal ion catalysis in the Tetrahymena ribozyme reaction

ALL catalytic RNAs (ribozymes) require or are stimulated by divalent metal ions, but it has been difficult to separate the contribution of these metal ions to formation of the RNA tertiary structure¹ from a more ...

Joseph A. Piccirilli, Joseph S. Vyle, Marvin H. Caruthers, Thomas R. Cech in Nature (1993)

Article

RNA substrate binding site in the catalytic core of the Tetrahymena ribozyme

In catalysis by group I introns, the helix (P1) containing the RNA cleavage site must be positioned next to the guanosine binding site. We have identified a conserved adenine in the catalytic core that contrib...

Anna Marie Pyle, Felicia L. Murphy, Thomas R. Cech in Nature (1992)

Article

Inhibition of telomerase by G-quartet DMA structures

THE ends or telomeres of the linear chromosomes of eukaryotes are composed of tandem repeats of short DNA sequences, one strand being rich in guanine (G strand) and the complementary strand in cytosine^1,2. Telome...

Alan M. Zahler, James R. Williamson, Thomas R. Cech, David M. Prescott in Nature (1991)

Article

Ribozyme recognition of RNA by tertiary interactions with specific ribose 2′-OH groups

SHORTENED forms of the group I intron from Tetrahymena catalyse sequence-specific cleavage of exogenous oligonucleotide substrates^1,2. The association between RNA enzyme (ribozyme) and substrate is mediated by pa...

Anna Marie Pyle, Thomas R. Cech in Nature (1991)

Article

Self-splicing and enzymatic activity of an intervening sequence RNA fromTetrahymena

Thomas R. Cech in Bioscience Reports (1990)

Article

DNA cleavage catalysed by the ribozyme from Tetrahymena

An RNA enzyme derived from the self-splicing intervening sequence of Tetrahymena thermophila catalyses sequence-specific cleavage of an oligodeoxyribonucleotide substrate. Compared with RNA, the DNA substrate is ...

Daniel Herschlag, Thomas R. Cech in Nature (1990)

Article

Specificity for 3′,5′-linked substrates in RNA-catalyzed RNA polymerization

The finding that ribozymes can catalyze RNA chain elongation has led to the proposal that an early self-replicating system could have consisted of RNA alone. In such a chain elongation reaction, theTetrahymena ri...

Benjamin Young, Thomas R. Cech in Journal of Molecular Evolution (1989)

Article

Ribozyme self-replication?

Thomas R. Cech in Nature (1989)

Article

G-strings at chromosome ends

Thomas R. Cech in Nature (1988)

Article

The Tetrahymena ribozyme acts like an RNA restriction endonuclease

A shortened form of the Tetrahymena self-splicing ribosomal RNA intervening sequence acts as an endoribonuclease, catalysing the cleavage of large RNA molecules by a mechanism involving guanosine transfer. The...

Arthur J. Zaug, Michael D. Been, Thomas R. Cech in Nature (1986)

Article

Mechanism of recognition of the 5′ splice site in self-splicing group I introns

Group I introns include many mitochondrial ribosomal RNA and messenger RNA introns and the nuclear rRNA introns of Tetrahymena and Physarum^1–6. The splicing of precursor RNAs containing these introns is a two-ste...

Gian Garriga, Alan M. Lambowitz, Tan Inoue, Thomas R. Cech in Nature (1986)

Chapter

Conformational Dynamics Involved in RNA Self-Splicing

In some species of the ciliated protozoan, Tetrahymena, the genes for the large rRNA (ribosomal RNA) are interrupted by an IVS (intervening sequence or intron) approximately 400 base pairs in size (Wild and Gall,...

Thomas R. Cech, Francis X. Sullivan, Tan Inoue… in Structure and Dynamics of RNA (1986)

Article

Specific interaction between the self-splicing RNA of Tetrahymena and its guanosine substrate: implications for biological catalysis by RNA

Splicing of the ribosomal RNA precursor of Tetrahymena has previously been shown to require no protein in vitro; the cleavage–ligation activity is intrinsic to the RNA molecule. Analysis of the reaction kinetics ...

Brenda L. Bass, Thomas R. Cech in Nature (1984)