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Article
Levan and fructosyl derivatives formation by a recombinant levansucrase from Rahnella aquatilis
Levan, fructo-oligosaccharides and fructosyl derivatives were formed from sucrose using recombinant levansucrase from Rahnella aquatilis. Levan formation was optimal at 30 °C resulting 57 % of the theoretical ...
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Article
Eicosapentaenoic acid (EPA) biosynthetic gene cluster ofShewanella oneidensis MR-1: Cloning, heterologous expression, and effects of temperature and glucose on the production of EPA inEscherichia coli
The putative EPA synthesis gene cluster was mined from the entire genome sequence ofShewanella oneidensis MR-1. The gene cluster encodes a PKS-like pathway that consists of six open reading frames (ORFs): ORFSO16...
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Article
Effect of salt concentration on production of polyunsaturated fatty acids in Thraustochytrium aureum ATCC 34304
This study attempted to characterize the effects of NaCl and MgSO4 as the primary components of sea salt affecting the yields of production of polyunsaturated fatty acids (PUFAs) by Thraustochytrium aureum ATCC 3...
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Article
Identification of Δ9-elongation activity from Thraustochytrium aureum by heterologous expression in Pichia pastoris
The Δ9-elongase isolated from Thraustochytrium aureum, which contains a high level of polyunsaturated fatty acids (PUFAs), was demonstrated to be associated with the synthesis of C20 PUFAs. The TaELO gene contain...
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Article
Optimization of culture conditions for 1,3-propanediol production from crude glycerol by Klebsiella pneumoniae using response surface methodology
To produce 1,3-propanediol (1,3-PD) from crude glycerol, cultivation conditions were optimized by response surface methodology (RSM) based on a 25 factorial central composite design (CCD). RSM was adopted to deri...
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Article
Enhancement of heterologous production of eicosapentaenoic acid in Escherichia coli by substitution of promoter sequences within the biosynthesis gene cluster
To enhance the heterologous production of eicosapentaenoic acid (EPA) in Escherichia coli, the EPA biosynthesis gene cluster from Shewanella oneidensis MR-1 was cloned under the lacZ promoter on a high-copy numbe...
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Article
Bacterial expression and purification of human papillomavirus type 18 L1
The human papillomavirus (HPV) 18 L1 gene, which encodes the L1 major capsid protein, was isolated from a female patient in Pusan, Korea Republic and was cloned into pGEX-4T-1 vector. The HPV-18L1 gene was exp...
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Article
An efficient plasmid vector for constitutive high-level expression of foreign genes in Escherichia coli
The levansucrase gene (lsrA) from Rahnella aquatilis was strongly expressed in a constitutive manner in Escherichia coli when cloned into a pBluescript KS-based pRL1CP plasmid vector. The native promoter upstream...
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Article
Elimination of by-product formation during production of 1,3-propanediol in Klebsiella pneumoniae by inactivation of glycerol oxidative pathway
The microbial production of 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae involves the formation of various by-products, which are synthesized through the oxidative pathway. To eliminate the by-products synth...
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Article
Improved ethanol tolerance in Escherichia coli by changing the cellular fatty acids composition through genetic manipulation
To investigate the effect of cellular fatty acids composition on ethanol tolerance in Escherichia coli, we overexpressed either des, encoding fatty acid desaturase from Bacillus subtilis, or fabA, encoding β-hydr...
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Article
Identification and utilization of a 1,3-propanediol oxidoreductase isoenzyme for production of 1,3-propanediol from glycerol in Klebsiella pneumoniae
In a previous study, we showed that 1,3-propanediol (1,3-PD) was still produced from glycerol by the Klebsiella pneumoniae mutant strain defective in 1,3-PD oxidoreductase (DhaT), although the production level wa...
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Article
Gene cloning, characterization, and heterologous expression of levansucrase from Bacillus amyloliquefaciens
Although levan produced by Bacillus amyloliquefaciens is known to have efficient immunostimulant property which gives 100% survival of common carp when infected with Aeromonas hydrophila, no detailed reports are ...
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Article
Identification and characterization of a novel enzyme related to the synthesis of PUFAs derived from Thraustochytrium aureum ATCC 34304
In Thraustochytrids, Thraustochytrium aureum ATCC 34304 was able to produce high levels of several polyunsaturated fatty acids. In the present study, a novel gene encoding protein was cloned from the DHA rich mic...
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Article
Melanogenesis inhibitory effect of dehydroevodiamine isolated from fruits of Evodia rutaecarpa
Dehydroevodiamine, an alkaloid, was isolated from the fruit of Evodia rutaecarpa and melanin production, and tyrosinase inhibition in B16F10 melanoma cells treated with the isolated dehydroevodiamine was investig...
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Article
Enhanced production of ethanol from glycerol by engineered Hansenula polymorpha expressing pyruvate decarboxylase and aldehyde dehydrogenase genes from Zymomonas mobilis
To improve production of ethanol from glycerol, the methylotrophic yeast Hansenula polymorpha was engineered to express the pdc and adhB genes encoding pyruvate decarboxylase and aldehyde dehydrogenase II from Zy...
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Article
Characterization of alcohol dehydrogenase 1 of the thermotolerant methylotrophic yeast Hansenula polymorpha
The thermotolerant methylotrophic yeast Hansenula polymorpha has recently been gaining interest as a promising host for bioethanol production due to its ability to ferment xylose, glucose, and cellobiose at eleva...
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Article
1,3-Propandiol production by engineered Hansenula polymorpha expressing dha genes from Klebsiella pneumoniae
Currently, 1,3-propanediol (1,3-PD) is an important chemical widely used in polymer production, but its availability is being restricted owing to its expensive chemical synthesis. A methylotrophic yeast Hansenula...
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Article
Identification and characterization of the propanediol utilization protein PduP of Lactobacillus reuteri for 3-hydroxypropionic acid production from glycerol
Although the de novo biosynthetic mechanism of 3-hydroxypropionic acid (3-HP) in glycerol-fermenting microorganisms is still unclear, the propanediol utilization protein (PduP) of Lactobacillus species has been s...
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Article
Stimulation of reductive glycerol metabolism by overexpression of an aldehyde dehydrogenase in a recombinant Klebsiella pneumoniae strain defective in the oxidative pathway
Previously, we constructed a glycerol oxidative pathway-deficient mutant strain of Klebsiella pneumoniae by inactivation of glycerol dehydrogenase (dhaD) to eliminate by-product synthesis during production of 1,3...
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Article
Production of Lipids Containing High Levels of Docosahexaenoic Acid by a Newly Isolated Microalga, Aurantiochytrium sp. KRS101
In the present study, a novel oleaginous Thraustochytrid containing a high content of docosahexaenoic acid (DHA) was isolated from a mangrove ecosystem in Malaysia. The strain identified as an Aurantiochytrium sp...
