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Levan and fructosyl derivatives formation by a recombinant levansucrase from Rahnella aquatilis

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Abstract

Levan, fructo-oligosaccharides and fructosyl derivatives were formed from sucrose using recombinant levansucrase from Rahnella aquatilis. Levan formation was optimal at 30 °C resulting 57 % of the theoretical yield. The more suitable substrate concentration for levan formation was 200 g sucrose/L. Oligosaccharides was accumulated selectively at high substrate concentration. The increase of levan and oligosaccharides formation was not achieved by adding water-miscible organic solvents. Alkyl fructosides were synthesized from various alcohols as fructosyl acceptors by R. aquatilis levansucrase. © Rapid Science Ltd. 1998

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Authors and Affiliations

  1. Applied Microbiology Research Division, Korea Research Institute of Bioscience and Biotechnology, Yusong Taejon, Korea

    Min Gon Kim, Jeong Woo Seo, Ki-Bang Song, Chul-Ho Kim & Sang-Ki Rhee

  2. Bioprocess Technology Research Division, Korea Research Institute of Bioscience and Biotechnology, Yusong Taejon, Korea

    Bong Hyun Chung

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  1. Min Gon Kim
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  2. Jeong Woo Seo
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  3. Ki-Bang Song
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  4. Chul-Ho Kim
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  5. Bong Hyun Chung
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  6. Sang-Ki Rhee
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Kim, M.G., Seo, J.W., Song, KB. et al. Levan and fructosyl derivatives formation by a recombinant levansucrase from Rahnella aquatilis. Biotechnology Letters 20, 333–336 (1998). https://doi.org/10.1023/A:1005310926399

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