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Article
Organic solvent stable protease isolation and characterization from organic solvent tolerant strain of Lysinibacillus sphaericus PAP02
In this study, a bacterium producing solvent-stable protease was isolated from salt-enriched soil after incubation in benzene and toluene enriched-media and identified as Lysinibacillus sphaericus PAP02 by 16S rR...
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Article
Production of alkaline protease from a newly isolated Exiguobacterium profundum BK-P23 evaluated using the response surface methodology
Protease producing bacteria were isolated from soil in South Korea. These bacteria were screened in skim milk agar medium using skim milk as the substrate. The highest clear zone producing bacterial strain (BK...
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Article
The effects of culture condition on the growth property and docosahexaenoic acid production from Thraustochytrium aureum ATCC 34304
The effect of the composition of artificial sea water (ASW) medium on the growth properties and docosahexaenoic acid (DHA) production from Thraustochytrium aureum ATCC 34304 was investigated. A maximum dry cell w...
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Article
Growth of the oleaginous microalga Aurantiochytrium sp. KRS101 on cellulosic biomass and the production of lipids containing high levels of docosahexaenoic acid
We examined the growth of a novel oleaginous microalga, Aurantiochytrium sp. KRS101, using cellulosic materials as nutrients, and the resultant production of lipids containing high levels of docosahexaenoic acid ...
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Article
Production of Lipids Containing High Levels of Docosahexaenoic Acid by a Newly Isolated Microalga, Aurantiochytrium sp. KRS101
In the present study, a novel oleaginous Thraustochytrid containing a high content of docosahexaenoic acid (DHA) was isolated from a mangrove ecosystem in Malaysia. The strain identified as an Aurantiochytrium sp...
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Article
Identification and characterization of a novel enzyme related to the synthesis of PUFAs derived from Thraustochytrium aureum ATCC 34304
In Thraustochytrids, Thraustochytrium aureum ATCC 34304 was able to produce high levels of several polyunsaturated fatty acids. In the present study, a novel gene encoding protein was cloned from the DHA rich mic...
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Article
Isolation and identification of an anticancer drug, taxol from Phyllosticta tabernaemontanae, a leaf spot fungus of an angiosperm, Wrightia tinctoria
Phyllosticta tabernaemontanae, a leaf spot fungus isolated from the diseased leaves of Wrightia tinctoria, showed the production of taxol, an anticancer drug, on modified liquid medium (M...
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Article
Production of taxol from Phyllosticta dioscoreae, a leaf spot fungus isolated from Hibiscus rosa-sinensis
Taxol is a highly functionalized anticancer drug widely used in hospitals and clinics. The leaf spot fungus, Phyllosticta dioscoreae was isolated from diseased leaves of Hibiscus rosa-sinensis and screened for ex...
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Article
Enhancement of heterologous production of eicosapentaenoic acid in Escherichia coli by substitution of promoter sequences within the biosynthesis gene cluster
To enhance the heterologous production of eicosapentaenoic acid (EPA) in Escherichia coli, the EPA biosynthesis gene cluster from Shewanella oneidensis MR-1 was cloned under the lacZ promoter on a high-copy numbe...
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Article
Identification of Δ9-elongation activity from Thraustochytrium aureum by heterologous expression in Pichia pastoris
The Δ9-elongase isolated from Thraustochytrium aureum, which contains a high level of polyunsaturated fatty acids (PUFAs), was demonstrated to be associated with the synthesis of C20 PUFAs. The TaELO gene contain...
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Article
Lipid body formation by Thraustochytrium aureum (ATCC 34304) in response to cell age
The heterotrophic marine protist, Thraustochytrium aureum produces substantial amounts of polyunsaturated fatty acids (PUFAs). In the present investigation, changes in the lipid and fatty acid profiles of T. aure...
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Article
Coexpression of Elo-like enzyme and Δ5, Δ4-desaturases derived from Thraustochytrium aureum ATCC 34304 and the production of DHA and DPA in Pichia pastoris
Thraustochytrium aureum ATCC 34304 produces a high level of polyunsaturated fatty acids (PUFAs), which are typically synthesized by strings of reactions catalyzed by desaturase and elonga...
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Article
Investigation of the physiological properties and synthesis of PUFAs from Thraustochytrids and its electrophoretic karyotypes
Physiological properties of organism, such as the number of chromosomes, genome size, fatty acid profile and the activities of desaturases and elongases were investigated for four differentThraustochytrium specie...
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Article
Effect of salt concentration on production of polyunsaturated fatty acids in Thraustochytrium aureum ATCC 34304
This study attempted to characterize the effects of NaCl and MgSO4 as the primary components of sea salt affecting the yields of production of polyunsaturated fatty acids (PUFAs) by Thraustochytrium aureum ATCC 3...
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Article
Eicosapentaenoic acid (EPA) biosynthetic gene cluster ofShewanella oneidensis MR-1: Cloning, heterologous expression, and effects of temperature and glucose on the production of EPA inEscherichia coli
The putative EPA synthesis gene cluster was mined from the entire genome sequence ofShewanella oneidensis MR-1. The gene cluster encodes a PKS-like pathway that consists of six open reading frames (ORFs): ORFSO16...
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Article
Optimization of the lowry method of protein precipitation from theH. influenzae type b conjugate vaccine using deoxycholic acid and hydrochloric acid
The Lowry method was used in this study to measure protein inHaemophilus influenzae type b (Hib) conjugate vaccines (polyribosylibitol phosphate-tetanus toxoid; PRP-TT) using deoxycholic acid (DOC) to induce prot...
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Article
The growth and EPA synthesis ofShewanella oneidensis MR-1 and expectation of EPA biosynthetic pathway
Shewanella oneidensis MR-1 has the ability to inhale certain metals and chemical compounds and exhale these materials in an altered state; as a result, this microorganism has been widely ...
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Article
Importance of the degree of antigen polymerization by detoxification in modulating the immunogenicity of acellular pertussis vaccine
For the acellular pertussis vaccine with a high immunogenicity, the concentration, composition and characteristics of acellular pertussis antigens are the crucial points to be considered. Nevertheless, it has ...
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Article
An animal model to evaluate the protective efficacy ofHaemophilus influenzae type b conjugate vaccines
An efficacy test of PRP (polyribosylribitol phosphate)-TT (Tetanus toxoid) conjugate vaccines was carried out using BALB/c mice as an animal model by inoculatingHaemophilus influenzae type b (Hib) with a virulenc...
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Article
A numerical study on heat transfer characteristics in a spray column direct contact heat exchanger
A reliable computational heat transfer model has been investigated to define the heat transfer characteristics of a spray column direct contact heat exchanger, which is often utilized in the process involving ...
