Abstract
The levansucrase gene (lsrA) from Rahnella aquatilis was strongly expressed in a constitutive manner in Escherichia coli when cloned into a pBluescript KS-based pRL1CP plasmid vector. The native promoter upstream of lsrA and the lacZ promoter cooperatively enhanced the expression of lsrA to a level that was comparable to that of the T7 promoter, which is used in commercial pET expression vector system. A putative rho-independent transcription termination signal downstream of lsrA was crucial for gene expression. This plasmid vector also proved to be applicable for efficient expression of other foreign genes in E. coli.
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This work was supported by a Grant from Korean Research Foundation and by a grant from KRIBB Research Initiative Program.
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Seo, JW., Hong, Wk., Rairakhwada, D. et al. An efficient plasmid vector for constitutive high-level expression of foreign genes in Escherichia coli . Biotechnol Lett 31, 877–881 (2009). https://doi.org/10.1007/s10529-009-9941-4
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DOI: https://doi.org/10.1007/s10529-009-9941-4