103 Result(s)
within
Zhe** Yu
Biotechnology
-
Article
New carotenoids from the thermophilic green sulfur bacterium Chlorobium tepidum: 1′,2′-dihydro-γ-carotene, 1′,2′-dihydrochlorobactene, and OH-chlorobactene glucoside ester, and the carotenoid composition of different strains
The complete carotenoid composition of the thermophilic green sulfur bacterium Chlorobium tepidum strain TNO was determined by spectroscopic methods. Major carotenoids were four kinds of carotenes: γ-carotene, c...
-
Article
Polyethylene glycol improves the purification of recombinant human tumor necrosis factor during ion exchange chromatography
Poly(ethylene glycol) (PEG) – either PEG 200, 600 or 4000 – when added to the buffer and feed solution gave nearly one-fold increase in the purification and about 100% recovery of recombinant human tumor necro...
-
Article
Antioxidative activity of green tea catechin extract compared with that of rosemary extract
This study compared the antioxidative activity of green tea catechin (GTC) extract with that of rosemary extract in canola oil, pork lard, and chicken fat. The GTC extract was obtained from jasmine and long**...
-
Article
Antioxidative activity of green tea catechin extract compared with that of rosemary extract
This study compared the antioxidative activity of green tea catechin (GTC) extract with that of rosemary extract in canola oil, pork lard, and chicken fat. The GTC extract was obtained from jasmine and long**...
-
Article
High level expression of a mutant (K151E, R154G) of single chain urokinase-type plasminogen activator in silkworm
A cDNA of a mutant (K151E, R154G) of single chain urokinase-type plasminogen activator (mscu-PA) was constructed to include the natural scu-PA signal peptide sequences and transferred into the genome of Bombyx...
-
Article
Effect of baicalein and acetone extract of Scutellaria baicalensis on canola oil oxidation
There is an increasing interest in natural antioxidants present in traditional Chinese herbal medicines. The present study examined the antioxidant activity of heane, acetone, and methanol extracts, as well as...
-
Article
Antioxidant activity of flavonoids isolated from Scutellaria rehderiana
The present study examined the antioxidant activity in heated canola oil of hexane, acetone, and methanol extracts of dry roots of gansu huangqin (Scutellaria rehderiana) as well as six flavonoids isolated from t...
-
Article
Novel hydroxycarotenoids with improved antioxidative properties produced by gene combination in Escherichia coli
We have used combinatorial biosynthesis to synthesize novel lipophilic carotenoids that are powerful cellular antioxidants. By co-expressing three different carotenoid desaturases in combination with a caroten...
-
Article
Repeated elicitation enhances taxane production in suspension cultures of Taxus chinensis in bioreactors
Methyl jasmonate added at 100 μM on days 8, 14 and 20 to cultures of Taxus chinensis growing in flasks and 1 l airlift bioreactors improved taxuyunnanine C (TC) production by 50–60% in flasks and by about 80% in ...
-
Article
Antioxidant activity of tea theaflavins and methylated catechins in canola oil
The present study examined the antioxidant activity of black tea theaflavins and catechin derivatives in canola oil. Oxidation was conducted at 95°C by monitoring the oxygen consumption and decreases in the li...
-
Reference Work Entry In depth
3D Structure Determination by NMR
-
Article
Salicylic Acid Enhances Jaceosidin and Syringin Production in Cell Cultures of Saussurea medusa
Addition of 20 μM salicylic acid to Saussurea medusa cell cultures at day 6 resulted in jaceosidin and syringin productions up to 95 mg l−1 and 631 mg l−1 which were, respectively, about ...
-
Article
Aspergillus niger lipase: gene cloning, over-expression in Escherichia coli and in vitro refolding
From the N-terminal amino acid sequence of the lipase from Aspergillus niger F044, a potential homologous gene A84689 to the lipanl (the gene encoding the lipase from Aspergillus niger F044) was identified. A pai...
-
Chapter
SNPs in microRNA and microRNA Target Sites Associated with Human Cancers
MicroRNAs (miRNAs) are small non-coding RNAs that play an important role in gene regulatory network. They regulate gene expression at the post-transcriptional level by base-pairing to the 3′-untraslated region...
-
Article
Simultaneous biological removal of sulfur, nitrogen and carbon using EGSB reactor
High-rate biological conversion of sulfide and nitrate in synthetic wastewater to, respectively, elemental sulfur (S0) and nitrogen-containing gas (such as N2) was achieved in an expanded granular sludge bed (EGS...
-
Article
Microbial community of granules in expanded granular sludge bed reactor for simultaneous biological removal of sulfate, nitrate and lactate
This study studied the cultivation of granules from an expanded granular sludge bed reactor that simultaneously transforms sulfates, nitrates, and oxygen to elementary sulfur, nitrogen gas, and carbon dioxides...
-
Article
afsQ1-Q2-sigQ is a pleiotropic but conditionally required signal transduction system for both secondary metabolism and morphological development in Streptomyces coelicolor
Two-component system AfsQ1-Q2 of Streptomyces coelicolor was identified previously for its ability to stimulate actinorhodin (ACT) and undecylprodigiosin (RED) production in Streptomyces lividans. However, disrup...
-
Article
Effect of anaerobic promoters on the microaerobic production of polyhydroxybutyrate (PHB) in recombinant Escherichia coli
Nine anaerobic promoters were cloned and constructed upstream of PHB synthesis genes phbCAB from Ralstonia eutropha for the micro- or anaerobic PHB production in recombinant Escherichia coli. Among the promoters,...
-
Article
A large number of tetraploid Arabidopsis thaliana lines, generated by a rapid strategy, reveal high stability of neo-tetraploids during consecutive generations
Arabidopsis thaliana has, in conjunction with A. arenosa, developed into a system for the molecular analysis of alloplolyploidy. However, there are very few Arabidopsis lines available to...
-
Article
Could nested PCR be applicable for the study of microbial diversity?
PCR-based methods for rRNA gene analysis have been widely used to study diversity of microbiology. However, the analysis would be difficult when the DNA content in samples is too low to be amplified by convent...
