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Article
Expression, refolding, and characterization of recombinant thrombopoietin/stem cell factor fusion protein in Escherichia coli
Thrombopoietin/stem cell factor (TPO/SCF) is a novel fusion protein that combines the complementary biological effects of TPO and SCF into a single molecule. In this study, TPO/SCF gene was cloned into pET32a ...
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Article
High-level expression of recombinant human paraoxonase 1 Q in silkworm larvae (Bombyx mori)
Human serum paraoxonase 1 (hPON1) belongs to a family of enzymes that catalyze the hydrolysis of a broad range of esters and lactones. Although the very first identification of hPON1 might have been as a calci...
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Article
Design of Recombinant Stem Cell Factor–macrophage Colony Stimulating Factor Fusion Proteins and their Biological Activity In Vitro
Stem cell factor (SCF) and macrophage colony stimulating factor (M-CSF) can act in synergistic way to promote the growth of mononuclear phagocytes. SCF–M-CSF fusion proteins were designed on the computer using...
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Article
Experimental proof for the regulation ofSalmonella typhimurium purB bypurR
Salmonella typhimurium purB encodes adenylosuccinate lyase (ASL), the enzyme that catalyzes step 8 in the pathway forde novo synthesis of inosine 5′-monophosphate (IMP) and also the final...
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Article
High level expression of a mutant (K151E, R154G) of single chain urokinase-type plasminogen activator in silkworm
A cDNA of a mutant (K151E, R154G) of single chain urokinase-type plasminogen activator (mscu-PA) was constructed to include the natural scu-PA signal peptide sequences and transferred into the genome of Bombyx...