6 Result(s)
within
Terrance A Stadheim
Biotechnology
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Article
Elimination of diaminopeptidase activity in Pichia pastoris for therapeutic protein production
Yeast are important production platforms for the generation of recombinant proteins. Nonetheless, their use has been restricted in the production of therapeutic proteins due to differences in their glycosylati...
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Article
Erratum to: Improvement of N-glycan site occupancy of therapeutic glycoproteins produced in Pichia pastoris
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Article
Improvement of N-glycan site occupancy of therapeutic glycoproteins produced in Pichia pastoris
Yeast is capable of performing posttranslational modifications, such as N- or O-glycosylation. It has been demonstrated that N-glycans play critical biological roles in therapeutic glycoproteins by modulating pha...
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Article
Open AccessGeneration of diploid Pichia pastoris strains by mating and their application for recombinant protein production
Yeast mating provides an efficient means for strain and library construction. However, biotechnological applications of mating in the methylotrophic yeast Pichia pastoris have been hampered because of concerns ab...
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Article
High-throughput screening and selection of yeast cell lines expressing monoclonal antibodies
The methylotrophic yeast Pichia pastoris has recently been engineered to express therapeutic glycoproteins with uniform human N-glycans at high titers. In contrast to the current art where producing therapeutic p...
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Article
Optimization of humanized IgGs in glycoengineered Pichia pastoris
As the fastest growing class of therapeutic proteins, monoclonal antibodies (mAbs) represent a major potential drug class1. Human antibodies are glycosylated in their native state and all clinically approved mAbs...
