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Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production

Yeast mating provides an efficient means for strain and library construction. However, biotechnological applications of mating in the methylotrophic yeast Pichia pastoris have been hampered because of concerns ab...

Ming-Tang Chen, Song Lin, Ishaan Shandil, Dewan Andrews… in Microbial Cell Factories (2012)