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    Article

    A practical approach for O-linked mannose removal: the use of recombinant lysosomal mannosidase

    The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation. Advances in glycoengineering o...

    Daniel Hopkins, Sujatha Gomathinayagam… in Applied Microbiology and Biotechnology (2015)

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    Article

    In vitro enzymatic treatment to remove O-linked mannose from intact glycoproteins

    The methylotrophic yeast Pichia pastoris is an attractive expression system for heterologous protein production due to its ability to perform posttranslational modifications, such as glycosylation, and secrete la...

    Sujatha Gomathinayagam, Stephen R. Hamilton in Applied Microbiology and Biotechnology (2014)

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    Article

    Elimination of diaminopeptidase activity in Pichia pastoris for therapeutic protein production

    Yeast are important production platforms for the generation of recombinant proteins. Nonetheless, their use has been restricted in the production of therapeutic proteins due to differences in their glycosylati...

    Daniel Hopkins, Sujatha Gomathinayagam… in Applied Microbiology and Biotechnology (2014)

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    Article

    The Impact of Glycosylation on the Pharmacokinetics of a TNFR2:Fc Fusion Protein Expressed in Glycoengineered Pichia Pastoris

    P. pastoris has previously been genetically engineered to generate strains that are capable of producing mammalian-like glycoforms. Our objective was to investigate the correlation bet...

    Liming Liu, Sujatha Gomathinayagam, Lora Hamuro… in Pharmaceutical Research (2013)

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    High-Throughput Multimodal Strong Anion Exchange Purification and N-Glycan Characterization of Endogenous Glycoprotein Expressed in Glycoengineered Pichia pastoris

    The secretory pathway of the yeast Pichia pastoris has been engineered to produce complex human-type N-glycans (Choi et al., Proc Natl Acad Sci USA 100:5022–5027, 2003; Hamilton et al., Science 301:1244–1246, 200...

    Sujatha Gomathinayagam, Erik Hoyt, Alissa M. Thompson, Eric Brown… in Therapeutic Proteins (2012)