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Article
Open AccessEvaluating digestion efficiency in full-scale anaerobic digesters by identifying active microbial populations through the lens of microbial activity
Anaerobic digestion is a common technology to biologically stabilize wasted solids produced in municipal wastewater treatment. Its efficiency is usually evaluated by calculating the reduction in volatile solid...
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Protocol
Primers: Functional Genes and 16S rRNA Genes for Methanogens
To date, a great number of oligonucleotide probes/primers targeting phylogenetic markers of methanogenic archaea (methanogens), such as 16S rRNA and the gene for the α-subunit of methyl-coenzyme M reductase (mcrA
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Article
Analysis of microbiota involved in the aged natural fermentation of indigo
Although the indigo reduction process is performed via natural fermentation and maintained under open-air condition, the indigo-reducing reactions continue for 6 months (on average) or longer. Identifying the ...
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Article
Response of Propionate-Degrading Methanogenic Microbial Communities to Inhibitory Conditions
Propionate is a crucial intermediate during methane fermentation. Investigating the effects of different kinds of inhibitors on the propionate-degrading microbial community is necessary to develop countermeasu...
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Article
Identification of novel potential acetate-oxidizing bacteria in thermophilic methanogenic chemostats by DNA stable isotope probing
Syntrophic oxidization of acetate and propionate are both critical steps of methanogenesis during thermophilic anaerobic digestion. However, knowledge on syntrophic acetate-oxidizing bacteria (SAOB) and syntro...
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Article
Open AccessUsing DNA-based stable isotope probing to reveal novel propionate- and acetate-oxidizing bacteria in propionate-fed mesophilic anaerobic chemostats
Propionate is one of the most important intermediates of anaerobic fermentation. Its oxidation performed by syntrophic propionate-oxidizing bacteria coupled with hydrogenotrophic methanogens is considered to b...
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Article
Different Interspecies Electron Transfer Patterns during Mesophilic and Thermophilic Syntrophic Propionate Degradation in Chemostats
Propionate is one of the major intermediates in anaerobic digestion of organic waste to CO2 and CH4. In methanogenic environments, propionate is degraded through a mutualistic interaction between symbiotic propio...
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Article
Open AccessCatabolism and interactions of uncultured organisms shaped by eco-thermodynamics in methanogenic bioprocesses
Current understanding of the carbon cycle in methanogenic environments involves trophic interactions such as interspecies H2 transfer between organotrophs and methanogens. However, many metabolic processes are th...
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Article
Early prediction of environmental deterioration in a coastal fish farming area using lactic acid bacteria as an indicator
We hypothesized that lactic acid bacteria (LAB) present in sediments at aquaculture sites can be employed as an indicator for the early prediction of deterioration, as the concentration of organic acids contro...
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Article
Corallococcus caeni sp. nov., a novel myxobacterium isolated from activated sludge
Two myxobacterial strains (KH5-1T and NO1) were isolated from the activated sludge tanks treating municipal sewage wastewater in Japan. These strains were recognised as myxobacteria based on their phenotypic char...
