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Article
Response of Isovalerate-Degrading Methanogenic Microbial Community to Inhibitors
Isovalerate is one of the key intermediates during anaerobic digestion treating protein-containing waste/wastewater. Investigating the effect of different kinds of inhibitors on isovalerate-degrading microbial...
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Article
Different Interspecies Electron Transfer Patterns during Mesophilic and Thermophilic Syntrophic Propionate Degradation in Chemostats
Propionate is one of the major intermediates in anaerobic digestion of organic waste to CO2 and CH4. In methanogenic environments, propionate is degraded through a mutualistic interaction between symbiotic propio...
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Article
Open AccessUsing DNA-based stable isotope probing to reveal novel propionate- and acetate-oxidizing bacteria in propionate-fed mesophilic anaerobic chemostats
Propionate is one of the most important intermediates of anaerobic fermentation. Its oxidation performed by syntrophic propionate-oxidizing bacteria coupled with hydrogenotrophic methanogens is considered to b...
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Article
Identification of novel potential acetate-oxidizing bacteria in thermophilic methanogenic chemostats by DNA stable isotope probing
Syntrophic oxidization of acetate and propionate are both critical steps of methanogenesis during thermophilic anaerobic digestion. However, knowledge on syntrophic acetate-oxidizing bacteria (SAOB) and syntro...
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Article
Response of Propionate-Degrading Methanogenic Microbial Communities to Inhibitory Conditions
Propionate is a crucial intermediate during methane fermentation. Investigating the effects of different kinds of inhibitors on the propionate-degrading microbial community is necessary to develop countermeasu...
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Article
Long-term effects of heavy metals and antibiotics on granule-based anammox process: granule property and performance evolution
The feasibility of the anaerobic ammonium oxidation (anammox) process to treat synthetic swine wastewater containing antibiotics and heavy metals was studied in this work. Nitrogen removal performance and gran...
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Article
Analyzing the revolution of anaerobic ammonium oxidation (anammox) performance and sludge characteristics under zinc inhibition
In the present study, the short- and long-term effects of Zn(II) on the anaerobic ammonium oxidation (anammox) performance and sludge characteristics were evaluated. The anammox activity decreased with increas...
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Article
Overexpression of DnWRKY11 enhanced salt and drought stress tolerance of transgenic tobacco
Dendrobium seedlings showed low survival rate when they were transferred from in vitro conditions to greenhouse or field environment. One of the major reasons is their low tolerance to environmen...
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Article
Molecular Phylogeny Analysis and Species Identification of Dendrobium (Orchidaceae) in China
Dendrobium plants are important commercial herbs in China, widely used in traditional medicine and ornamental horticulture. In this study, sequence-related amplified polymorphism (SRAP) markers w...
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Article
Thirteen novel polymorphic microsatellite markers for endangered Chinese endemic herb Dendrobium Officinale
We isolated and developed 13 novel microsatellite makers from expressed sequence tag sequences of endangered Chinese endemic herb Dendrobium officinale and examined them for 38 individuals from Ji...
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Article
Open AccessHigh codon adaptation in citrus tristeza virus to its citrus host
Citrus tristeza virus (CTV), a member of the genus Closterovirus within the family Closteroviridae, is the causal agent of citrus tristeza disease. Previous studies revealed that the negative selection, RNA recom...
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Article
Genetic variation and cultivar identification in Cymbidium ensifolium
As a popular flowering species with many cultivars, Cymbidium ensifolium (L.) is commercially important in horticulture. However, so far little has been known about genetic diversity and conservation genetics of ...
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Article
Molecular diversity and relationships among Cymbidium goeringii cultivars based on inter-simple sequence repeat (ISSR) markers
Spring orchid (Cymbidium goeringii) is a popular flowering plant species. There have been few molecular studies of the genetic diversity and conservation genetics on this species. An assessment of the level of ge...
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Article
Application of Arabidopsis AGAMOUS second intron for the engineered ablation of flower development in transgenic tobacco
To explore a new approach to generating reproductive sterility in transgenic plants, the barnase gene from Bacillus amyloliquefaciens was placed under the control of an 1853-bp nucleotide sequence from the 3′end ...
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Article
Co-transformation of gene expression cassettes via particle bombardment to generate safe transgenic plant without any unwanted DNA
The presence of resistant selectable marker genes and other added DNAs such as the vector backbone sequence in transgenic plant might be an unpredictable hazard to the ecosystem as well as to human health, whi...