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Article
Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants
The number and diversity of known CRISPR–Cas systems have substantially increased in recent years. Here, we provide an updated evolutionary classification of CRISPR–Cas systems and cas genes, with an emphasis on ...
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Chapter
Archaeal Viruses and Their Interactions with CRISPR-Cas Systems
Our knowledge of archaeal viruses has increased rapidly over the past four decades since the discovery of the archaeal domain. Most surprising has been the morphological diversity of crenarchaeal viruses that ...
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Article
Archaeal physiology: The secrets of termination
Regulation of transcriptional termination in archaea has remained a mystery. Now, a high-throughput RNA sequencing approach identifies multiple archaeal genes that contain consecutive terminators, suggesting n...
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Article
An updated evolutionary classification of CRISPR–Cas systems
CRISPR–Cas systems provide archaea and bacteria with adaptive immunity against viruses and plasmids.
CRISPR...
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Protocol
Archaeal Viruses of the Sulfolobales: Isolation, Infection, and CRISPR Spacer Acquisition
Infection of archaea with phylogenetically diverse single viruses, performed in different laboratories, has failed to activate spacer acquisition into host CRISPR loci. The first successful uptake of archaeal ...
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Article
Open AccessAdenosine triphosphatases of thermophilic archaeal double-stranded DNA viruses
Adenosine triphosphatases (ATPases) of double-stranded (ds) DNA archaeal viruses are structurally related to the AAA+ hexameric helicases and translocases. These ATPases have been implicated in viral life cycl...
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Article
A novel single-tailed fusiform Sulfolobus virus STSV2 infecting model Sulfolobus species
A newly isolated single-tailed fusiform virus, Sulfolobus tengchongensis spindle-shaped virus STSV2, from Hamazui, China, is characterised. It contains a double-stranded modified DNA genome of 76,107 bp and is en...
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Chapter
Archaeal Type II Toxin-Antitoxins
A few of the bacterial type II TA systems, primarily those involved in translational inhibition, occur widely throughout the archaeal domain. Using a bioinformatic approach, the frequency and distribution of t...
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Chapter
Discovery and Seminal Developments in the CRISPR Field
In the late 1980s and early 1990s, arrays of regularly spaced repeats were detected in both bacterial and archaeal genomes. They are currently known as Clustered Regularly Interspaced Short Palindromic Repeats...
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Article
Open AccessArchaeal viruses—novel, diverse and enigmatic
Recent research has revealed a remarkable diversity of viruses in archaeal-rich environments where spindles, spheres, filaments and rods are common, together with other exceptional morphotypes never recorded p...
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Article
Open AccessThe expression of one ankyrin pk2 allele of the WO prophage is correlated with the Wolbachia feminizing effect in isopods
The maternally inherited α-Proteobacteria Wolbachia pipientis is an obligate endosymbiont of nematodes and arthropods, in which they induce a variety of reproductive alterations, including Cytoplasmic Incompatibi...
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Chapter
CRISPR/Cas and CRISPR/Cmr Immune Systems of Archaea
The CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/ CRISPR-Associated Genes) and CRISPR/Cmr systems (Cmr: Cas module-RAMP (Repeat-Associated Mysterious Proteins)) provide the basis for a...
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Article
Open AccessGenomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles
The Acidianus hospitalis W1 genome consists of a minimally sized chromosome of about 2.13 Mb and a conjugative plasmid pAH1 and it is a host for the model filamentous lipothrixvirus AFV1. The chromosome carries t...
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Article
Open AccessThe Scottish Structural Proteomics Facility: targets, methods and outputs
The Scottish Structural Proteomics Facility was funded to develop a laboratory scale approach to high throughput structure determination. The effort was successful in that over 40 structures were determined. T...
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Article
Viruses of the Archaea: a unifying view
So far, all characterized archaeal viruses carry dsDNA genomes and exhibit a wide range of virion morphotypes, strongly surpassing the dsDNA viruses of the Bac...
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Article
Independent virus development outside a host
Growing two long filamentous tails may help an archaeal virus to survive in a hostile environment.
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Article
Gene capture in archaeal chromosomes
Free genetic elements can be readily integrated into bacterial chromosomes, but so far, with the exception of one virus, there has been no evidence that this happens in Archaea — the other domain of microorgan...
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Article
Completing the sequence of the Sulfolobus solfataricus P2 genome
The Sulfolobus solfataricus P2 genome collaborators are poised to sequence the entire 3-Mbp genome of this crenarchaeote archaeon. About 80% of the genome has been sequenced to date, with the rest of the sequenc...
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Protocol
Chemical and Enzymatic Probing of Antibiotic-Ribosome Complexes
It has become clear over the past decade that many of the antibiotics that inhibit protein biosynthesis act at the level of rRNA. The earliest indications were the demonstrations that the host producers of som...
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Article
Secondary structural elements exclusive to the sequences flanking ribosomal RNAs lend support to the monophyletic nature of the archaebacteria
Several sequences flanking the large rRNA genes of several transcripts from extreme thermophiles, extreme halophiles, and methanogens were aligned and analyzed for the presence of common primary and secondary ...
