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Article
Open AccessExtracellular vesicles are the main contributor to the non-viral protected extracellular sequence space
Environmental virus metagenomes, commonly referred to as “viromes”, are typically generated by physically separating virus-like particles (VLPs) from the microbial fraction based on their size and mass. Howeve...
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Article
A trove of Asgard archaeal viruses
Three different studies identify Asgardarchaeota-linked virus genomes that share commonalities with both prokaryotic and eukaryotic viruses, yet they represent independent phylogenetic groups.
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Protocol
Isolation, Purification, and Characterization of Membrane Vesicles from Haloarchaea
Membrane vesicles (MVs), also described as extracellular vesicles (EVs), exosomes, or outer membrane vesicles (OMVs), are nano-sized (10–300 nm) spherical, membrane-bound structures deriving from the cell enve...
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Article
Open AccessGenomic variation and biogeography of Antarctic haloarchaea
The genomes of halophilic archaea (haloarchaea) often comprise multiple replicons. Genomic variation in haloarchaea has been linked to viral infection pressure and, in the case of Antarctic communities, can be...
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Article
A plasmid from an Antarctic haloarchaeon uses specialized membrane vesicles to disseminate and infect plasmid-free cells
The major difference between viruses and plasmids is the mechanism of transferring their genomic information between host cells. Here, we describe the archaeal plasmid pR1SE from an Antarctic species of haloar...
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Article
Open AccessRepression of RNA polymerase by the archaeo-viral regulator ORF145/RIP
Little is known about how archaeal viruses perturb the transcription machinery of their hosts. Here we provide the first example of an archaeo-viral transcription factor that directly targets the host RNA poly...
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Protocol
Archaeal Viruses of the Sulfolobales: Isolation, Infection, and CRISPR Spacer Acquisition
Infection of archaea with phylogenetically diverse single viruses, performed in different laboratories, has failed to activate spacer acquisition into host CRISPR loci. The first successful uptake of archaeal ...
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Article
Open AccessAdenosine triphosphatases of thermophilic archaeal double-stranded DNA viruses
Adenosine triphosphatases (ATPases) of double-stranded (ds) DNA archaeal viruses are structurally related to the AAA+ hexameric helicases and translocases. These ATPases have been implicated in viral life cycl...
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Article
A novel single-tailed fusiform Sulfolobus virus STSV2 infecting model Sulfolobus species
A newly isolated single-tailed fusiform virus, Sulfolobus tengchongensis spindle-shaped virus STSV2, from Hamazui, China, is characterised. It contains a double-stranded modified DNA genome of 76,107 bp and is en...
