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  1. No Access

    Protocol

    Image Analysis of DNA Fiber and Nucleus in Plants

    Advances in cytology have led to the application of a wide range of visualization methods in plant genome studies. Image analysis methods are indispensable tools where morphology, density, and color play impor...

    Nobuko Ohmido, Toshiyuki Wako, Seiji Kato… in Chromosome and Genomic Engineering in Plan… (2016)

  2. No Access

    Article

    Identification and Characterization of Novel Gypsy-Type Retrotransposons in a Biodiesel Crop, Jatropha curcas L

    Gypsy-type retrotransposons comprise a large proportion of the plant genome. Identification and determination of their chromosomal distribution could contribute to a better understanding of the r...

    Atefeh Alipour, Joyce A. Cartagena, Suguru Tsuchimoto… in Plant Molecular Biology Reporter (2014)

  3. No Access

    Article

    Visualization of the terminal structure of rice chromosomes 6 and 12 with multicolor FISH to chromosomes and extended DNA fibers

    High-resolution fluorescence in situ hybridization (FISH) on interphase and pachytene nuclei, and extended DNA fibers enabled microscopic distinction of DNA sequences less than a few thousands of base pairs apart...

    Nobuko Ohmido, Kyoko Kijima, Ikuo Ashikawa, J. Hans de Jong… in Plant Molecular Biology (2001)

  4. No Access

    Chapter

    Chromosome painting as a tool for rice genetics and breeding

    Chromosome painting and genomic in situ hybridization (GISH) are both effective methods for basic genetic research and practical breeding. These methods were applied even in the typically small chromosomes of ric...

    Rieko Shishido, Nobuko Ohmido, Kiichi Fukui in Chromosome Painting (2001)

  5. No Access

    Article

    Site-specific accumulation of a LINE-like retrotransposon in a sex chromosome of the dioecious plant Cannabis sativa

    Male-associated DNA sequences were analysed in hemp (Cannabis sativa L.), a dioecious plant with heteromorphic sex chromosomes. A male-associated DNA sequence in C. sativa (MADC1) and its flanking sequence encode...

    Koichi Sakamoto, Nobuko Ohmido, Kiichi Fukui, Hiroshi Kamada… in Plant Molecular Biology (2000)

  6. No Access

    Article

    Bryophyte 5S rDNA was inserted into 45S rDNA repeat units after the divergence from higher land plants

    The 5S ribosomal RNA genes (5S rDNA) are located independently from the 45S rDNA repeats containing 18S, 5.8S and 26S ribosomal RNA genes in higher eukaryotes. Southern blot and fluorescence in situ hybridization...

    Takefumi Sone, Masaki Fujisawa, Mizuki Takenaka, Saiko Nakagawa… in Plant Molecular Biology (1999)

  7. No Access

    Article

    Quantitative chromosome map of the polyploid Saccharum spontaneum by multicolor fluorescence in situ hybridization and imaging methods

    Somatic chromosomes of a wild relative of sugarcane (Saccharum spontaneum L.) anther culture-derived clone (AP 85-361, 2n=32) were identified and characterized by computer-aided imaging technology and molecula...

    Sen Ha, Paul H. Moore, Don Heinz, Seiji Kato, Nobuko Ohmido… in Plant Molecular Biology (1999)

  8. No Access

    Article

    Physical map** of unique nucleotide sequences on identified rice chromosomes

    A physical map** method for unique nucleotide sequences on specific chromosomal regions was developed combining objective chromosome identification and highly sensitive fluorescence in situ hybridisation (FI...

    Nobuko Ohmido, Yukio Akiyama, Kiichi Fukui in Plant Molecular Biology (1998)

  9. No Access

    Article

    Repetitive sequences: cause for variation in genome size and chromosome morphology in the genus Oryza

    Large variation in genome size as determined by the nuclear DNA content and the mitotic chromosome size among diploid rice species is revealed using flow cytometry and image analyses. Both the total chromosoma...

    Sakurako Uozu, Hiroshi Ikehashi, Nobuko Ohmido, Hisako Ohtsubo… in Plant Molecular Biology (1997)

  10. No Access

    Article

    Visual verification of close disposition between a rice A genome-specific DNA sequence (TrsA) and the telomere sequence

    A rice A genome-specific tandem repeat sequence (TrsA) and telomeric nucleotide sequences, (TTTAGGG)n, were simultaneously detected by multicolor fluorescence in situ hybridization (McFISH) using rice prometaphas...

    Nobuko Ohmido, Kiichi Fukui in Plant Molecular Biology (1997)

  11. No Access

    Article

    Chromosomal location and reorganization of the 45S and 5S rDNA in theBrachyscome lineariloba complex (Asteraceae)

    The chromosomal locations of the 45S (18S-5.8S-26S) and 5S ribosomal DNA in theBrachyscome lineariloba complex and two related species have been determined by the use of multicolor fluorescencein situ hybridizati...

    Junko Adachi, Kuniaki Watanabe, Kiichi Fukui, Nobuko Ohmido… in Journal of Plant Research (1997)