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Article
Open AccessVariational autoencoder-based chemical latent space for large molecular structures with 3D complexity
The structural diversity of chemical libraries, which are systematic collections of compounds that have potential to bind to biomolecules, can be represented by chemical latent space. A chemical latent space i...
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Article
Open AccessIdentification of the hypertension drug niflumic acid as a glycine receptor inhibitor
Glycine is one of the major neurotransmitters in the brainstem and the spinal cord. Glycine binds to and activates glycine receptors (GlyRs), increasing Cl− conductance at postsynaptic sites. This glycinergic syn...
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Article
Hes1 plays an essential role in Kras-driven pancreatic tumorigenesis
Most pancreatic ductal adenocarcinoma (PDAC) develops from pancreatic epithelial cells bearing activating mutant KRAS genes through precancerous lesions, i.e. acinar-to-ductal metaplasia (ADM) and pancreatic intr...
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Protocol
Method for Imaging Live-Cell RNA Using an RNA Aptamer and a Fluorescent Probe
Live-cell imaging of mRNA dynamics is increasingly important to understanding spatially restricted gene expression. We recently developed a convenient and versatile method that uses a gene-specific RNA aptamer...
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Article
Open AccessAdrenergic receptor agonists induce the differentiation of pluripotent stem cell-derived hepatoblasts into hepatocyte-like cells
Current induction methods of hepatocytes from human induced pluripotent stem cells (hiPSCs) are neither low cost nor stable. By screening a chemical library of 1,120 bioactive compounds and known drugs, we ide...
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Article
Open AccessImpact of extracellular matrix on engraftment and maturation of pluripotent stem cell-derived cardiomyocytes in a rat myocardial infarct model
Pluripotent stem cell-derived cardiomyocytes show great promise in regenerating the heart after myocardial infarction; however, several uncertainties exist that must be addressed before clinical trials. One pr...
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Article
VMAT2 identified as a regulator of late-stage β-cell differentiation
A screen for compounds that promote ES cell differentiation into pancreatic β cells identified a VMAT2- and monoamine-dependent suppression mechanism of pancreatic β-cell differentiation. VMAT2 inhibitors pote...
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Chapter and Conference Paper
Small Molecule Tools for Cell Biology and Cell Therapy
Up to the present time, bioactive small molecules have had three primary uses: as medicines, agrochemicals, and biological tools. During the past 10 years, our laboratory has been conducting research in tool d...
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Article
Mdm-2 binding and TAFII31 recruitment is regulated by hydrogen bond disruption between the p53 residues Thr18 and Asp21
Analyses of five wild-type p53 containing cell lines revealed lineage specific differences in phosphorylation of Thr18 after treatment with ionizing (IR) or ultraviolet (UV) radiation. Importantly, Thr18 phosp...
