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Protocol
Synthesis of Oligonucleotide–Peptide Conjugates for Biomedical and Technological Applications
Oligonucleotide–peptide conjugates have attracted considerable interest especially for biomedical uses. In the first part of this chapter, we describe protocols for the stepwise synthesis of oligonucleotides c...
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Article
Synthesis and properties of small interfering RNA duplexes carrying 5-ethyluridine residues
Oligoribonucleotides carrying 5-ethyluridine units were prepared using solid-phase phosphoramidite chemistry. The introduction of the tert-butyldimethylsilyl group at the 2′-OH position proceeded in good yield an...
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Chapter
Challenges and Opportunities for Oligonucleotide-Based Therapeutics by Antisense and RNA Interference Mechanisms
Oligonucleotide-based therapeutics may be one of the most promising approaches for the treatment of diseases. Although significant progress has been made in develo** these agents as drugs, several hurdles re...
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Article
An artificial DNAzyme RNA ligase shows a reaction mechanism resembling that of cellular polymerases
DNAzymes have become attractive due to their potential biomedical and biotechnological applications, as well as their advantages in terms of stability, efficiency and synthetic accessibility with respect to pr...
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Protocol
Dynamics-Function Analysis in Catalytic RNA Using NMR Spin Relaxation and Conformationally Restricted Nucleotides
A full understanding of biomolecular function requires an analysis of both the dynamic properties of the system of interest and the identification of those dynamics that are required for function. We describe ...
