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Article
Site-directed cysteine coupling of disulfide-containing non-antibody carrier proteins (THIOCAPs)
The development of a new generation of non-antibody protein drug delivery systems requires site-directed conjugation strategies to produce homogeneous, reproducible and scalable nanomedicines. For that, the ge...
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Article
Open AccessG-quadruplex binding properties of a potent PARP-1 inhibitor derived from 7-azaindole-1-carboxamide
Poly ADP-ribose polymerases (PARP) are key proteins involved in DNA repair, maintenance as well as regulation of programmed cell death. For this reason they are important therapeutic targets for cancer treatme...
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Protocol
Preparation of Lipid-Conjugated siRNA Oligonucleotides for Enhanced Gene Inhibition in Mammalian Cells
Nucleic acid conjugates are promising drugs for treating gene-related diseases. Conjugating specific units like lipids, cell-penetrating peptides, polymers, antibodies, and aptamers either at the 3′- or 5′-ter...
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Article
Open AccessA pH-dependent bolt involving cytosine bases located in the lateral loops of antiparallel G-quadruplex structures within the SMARCA4 gene promotor
Some lung and ovarian tumors are connected to the loss of expression of SMARCA4 gene. In its promoter region, a 44-nucleotides long guanine sequence prone to form G-quadruplex structures has been studied by me...
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Article
Open AccessCorrection: Corrigendum: The human mitochondrial transcription factor A is a versatile G-quadruplex binding protein
Scientific Reports 7: Article number: 43992; published online: 09 March 2017; updated: 06 April 2017 The original version of this Article contained an error in the spelling of the author Aleix Tarrés-Solé, whi...
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Article
Open AccessThe human mitochondrial transcription factor A is a versatile G-quadruplex binding protein
The ability of the guanine-rich strand of the human mitochondrial DNA (mtDNA) to form G-quadruplex structures (G4s) has been recently highlighted, suggesting potential functions in mtDNA replication initiation...
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Article
Sensitive and label-free detection of miRNA-145 by triplex formation
The development of new strategies for detecting microRNAs (miRNAs) has become a crucial step in the diagnostic field. miRNA profiles depend greatly on the sample and the analytical platform employed, leading s...
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Article
A Novel Formulation Based on 2,3-Di(tetradecyloxy)propan-1-amine Cationic Lipid Combined with Polysorbate 80 for Efficient Gene Delivery to the Retina
The aim of the present study was to evaluate the potential application of a novel formulation based on a synthesized cationic lipid 2,3-di(tetradecyloxy)propan-1-amine, combined with polysorbate 80 to deliver ...
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Chapter
Challenges and Opportunities for Oligonucleotide-Based Therapeutics by Antisense and RNA Interference Mechanisms
Oligonucleotide-based therapeutics may be one of the most promising approaches for the treatment of diseases. Although significant progress has been made in develo** these agents as drugs, several hurdles re...
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Article
Synthesis and in vitro inhibition properties of oligonucleotide conjugates carrying amphipathic proline-rich peptide derivatives of the sweet arrow peptide (SAP)
In this study, a series of derivatives of the amphipathic proline-rich sweet arrow peptide (SAP) were covalently linked to antisense oligonucleotides designed to inhibit Renilla luciferase gene. Oligonucleotide–p...
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Protocol
Functionalization of Surfaces with Synthetic Oligonucleotides
There is a large interest in the use of nucleic acids covalently bound to surfaces for a variety of biomedical uses: biosensors, microarrays, drug delivery, lab-on-chip devices, and gene therapy, etc. Most of ...
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Article
Synthesis and properties of small interfering RNA duplexes carrying 5-ethyluridine residues
Oligoribonucleotides carrying 5-ethyluridine units were prepared using solid-phase phosphoramidite chemistry. The introduction of the tert-butyldimethylsilyl group at the 2′-OH position proceeded in good yield an...
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Article
Synthesis and in vitro inhibition properties of siRNA conjugates carrying glucose and galactose with different presentations
Oligoribonucleotide conjugates and the corresponding siRNA duplexes against tumor necrosis factor carrying one, two, or four glucose and galactose residues at the 5′-end have been prepared using phosphoramidit...
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Article
Chemical equilibria studies using multivariate analysis methods
Chemical multiequilibria systems can be monitored efficiently with the aid of spectroscopic techniques. Both hard- and soft-modeling are effective and powerful tools to extract chemical information from spectr...
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Protocol
Synthesis of Oligonucleotide–Peptide Conjugates for Biomedical and Technological Applications
Oligonucleotide–peptide conjugates have attracted considerable interest especially for biomedical uses. In the first part of this chapter, we describe protocols for the stepwise synthesis of oligonucleotides c...
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Article
Stepwise synthesis of RNA conjugates carrying peptide sequences for RNA interference studies
Oligoribonucleotide conjugates carrying nuclear localization peptide sequences at the 3′-end were prepared stepwise on a single support. The siRNA duplex carrying the nuclear localization peptide sequence at t...
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Article
Solid-Phase Synthesis of Modified Oligonucleotides
Synthetic oligonucleotides are ubiquitously found in most laboratories since solid-phase synthesis protocols have become highly optimized. These protocols make it possible to synthesize a large variety of modi...
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Article
Transgenic Rice Plants Expressing the Antifungal AFP Protein from Aspergillus Giganteus Show Enhanced Resistance to the Rice Blast Fungus Magnaporthe Grisea
The Aspergillus giganteus antifungal protein (AFP), encoded by the afp gene, has been reported to possess in vitro antifungal activity against various economically important fungal pathogens, including the rice b...
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Article
Carbon nanotubes with DNA recognition
Since the discovery of their one-dimensional electronic band structure1, the leading candidate that has emerged for nanodevice applications is single-walled carbon nanotubes (SWNTs) . Here we unite their unique p...
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Article
Iodouracil-mediated photocrosslinking of DNA to EcoRII restriction endonuclease in catalytic conditions
We used a XeCl excimer laser with 50 ns pulses, a frequency of 0.3 Hz and a wavelength of 308 nm in appropriate conditions for the photocrosslinking of EcoRII restriction endonuclease to a 14-mer DNA duplex, cont...
