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Protocol
Dynamics-Function Analysis in Catalytic RNA Using NMR Spin Relaxation and Conformationally Restricted Nucleotides
A full understanding of biomolecular function requires an analysis of both the dynamic properties of the system of interest and the identification of those dynamics that are required for function. We describe ...
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Article
Open AccessThe 27 kDa Trypanosoma brucei Pentatricopeptide Repeat Protein is a G-tract Specific RNA Binding Protein
Pentatricopeptide repeat (PPR) proteins, a helical repeat family of organellar RNA binding proteins, play essential roles in post-transcriptional RNA processing. In Trypanosoma brucei, an expanded family of PPR p...
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Article
Alternate-site isotopic labeling of ribonucleotides for NMR studies of ribose conformational dynamics in RNA
Heteronuclear NMR spin relaxation studies of conformational dynamics are coming into increasing use to help understand the functions of ribozymes and other RNAs. Due to strong $$^{13}\hbox{C}--...
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Article
Improved distance analysis in RNA using network-editing techniques for overcoming errors due to spin diffusion
Multispin magnetization transfer, or spin diffusion, is a significant source of error in NOESY-derived distance measurements for the determination of nucleic acid solution structures. The BD-NOESY and CBD-NOES...
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Chapter
Approaches to the Determination of More Accurate Cross-Relaxation Rates and the Effects of Improved Distance Constraints on Protein Solution Structures
In the past decade, nuclear magnetic resonance (NMR) has become an accepted and widely-used technique for studying the structure and dynamics of small- to moderate-sized proteins, nucleic acids, oligosaccharid...