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Article
Open AccessG-quadruplex binding properties of a potent PARP-1 inhibitor derived from 7-azaindole-1-carboxamide
Poly ADP-ribose polymerases (PARP) are key proteins involved in DNA repair, maintenance as well as regulation of programmed cell death. For this reason they are important therapeutic targets for cancer treatme...
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Protocol
Dynamics-Function Analysis in Catalytic RNA Using NMR Spin Relaxation and Conformationally Restricted Nucleotides
A full understanding of biomolecular function requires an analysis of both the dynamic properties of the system of interest and the identification of those dynamics that are required for function. We describe ...
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Article
Open AccessA pH-dependent bolt involving cytosine bases located in the lateral loops of antiparallel G-quadruplex structures within the SMARCA4 gene promotor
Some lung and ovarian tumors are connected to the loss of expression of SMARCA4 gene. In its promoter region, a 44-nucleotides long guanine sequence prone to form G-quadruplex structures has been studied by me...
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Article
Sensitive and label-free detection of miRNA-145 by triplex formation
The development of new strategies for detecting microRNAs (miRNAs) has become a crucial step in the diagnostic field. miRNA profiles depend greatly on the sample and the analytical platform employed, leading s...
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Chapter
Challenges and Opportunities for Oligonucleotide-Based Therapeutics by Antisense and RNA Interference Mechanisms
Oligonucleotide-based therapeutics may be one of the most promising approaches for the treatment of diseases. Although significant progress has been made in develo** these agents as drugs, several hurdles re...
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Article
Synthesis and in vitro inhibition properties of siRNA conjugates carrying glucose and galactose with different presentations
Oligoribonucleotide conjugates and the corresponding siRNA duplexes against tumor necrosis factor carrying one, two, or four glucose and galactose residues at the 5′-end have been prepared using phosphoramidit...
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Protocol
Synthesis of Oligonucleotide–Peptide Conjugates for Biomedical and Technological Applications
Oligonucleotide–peptide conjugates have attracted considerable interest especially for biomedical uses. In the first part of this chapter, we describe protocols for the stepwise synthesis of oligonucleotides c...
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Article
Stepwise synthesis of RNA conjugates carrying peptide sequences for RNA interference studies
Oligoribonucleotide conjugates carrying nuclear localization peptide sequences at the 3′-end were prepared stepwise on a single support. The siRNA duplex carrying the nuclear localization peptide sequence at t...
