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Article
Open AccessExtra kinetic dimensions for label discrimination
Due to its sensitivity and versatility, fluorescence is widely used to detect specifically labeled biomolecules. However, fluorescence is currently limited by label discrimination, which suffers from the broad...
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Article
Open AccessHessian single-molecule localization microscopy using sCMOS camera
Single-molecule localization microscopy (SMLM) has the highest spatial resolution among the existing super-resolution imaging techniques, but its temporal resolution needs further improvement. An sCMOS camera ...
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Article
Open AccessLive cell single molecule-guided Bayesian localization super resolution microscopy
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Chapter and Conference Paper
WAR Bots: Combining Virtual and Augmented Realities for an Immersive and Enjoyable Gaming Experience
Despite the popularity of Augmented and Virtual Reality within gaming, the full potential of such technologies is yet to be fully taken advantage of. Thus, we introduce WAR Bots, a cyber-physical game which inten...
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Article
Open AccessReduced TRMU expression increases the sensitivity of hair-cell-like HEI-OC-1 cells to neomycin damage in vitro
Aminoglycosides are ototoxic to the cochlear hair cells and mitochondrial dysfunction is one of the major mechanisms behind ototoxic drug-induced hair cell death. TRMU (tRNA 5-methylaminomethyl-2-thiouridylate...
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Article
Open Access2-Aminoethoxydiphenyl Borate Potentiates CRAC Current by Directly Dilating the Pore of Open Orai1
2-Aminoethoxydiphenyl borate (2-APB) elicits potentiation current (Ip) on Ca2+ release-activated Ca2+ (CRAC) channels. An accurate investigation into this modulation mechanism would reveal how STIM1-dependent cha...
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Article
Open AccessBayesian localization microscopy based on intensity distribution of fluorophores
Super-resolution microscopy techniques have overcome the limit of optical diffraction. Recently, the Bayesian analysis of Bleaching and Blinking data (3B) method has emerged as an important tool to obtain supe...
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Article
Open AccessDrift correction for single-molecule imaging by molecular constraint field, a distance minimum metric
The recent developments of far-field optical microscopy (single molecule imaging techniques) have overcome the diffraction barrier of light and improve image resolution by a factor of ten compared with convent...
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Article
Open AccessThe specific and rapid labeling of cell surface proteins with recombinant FKBP-fused fluorescent proteins
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Article
Ultrafast, accurate, and robust localization of anisotropic dipoles
The resolution of single molecule localization imaging techniques largely depends on the precision of localization algorithms. However, the commonly used Gaussian function is not appropriate for anisotropic di...