-
Article
Readily releasable β cells with tight Ca2+–exocytosis coupling dictate biphasic glucose-stimulated insulin secretion
Biphasic glucose-stimulated insulin secretion (GSIS) is essential for blood glucose regulation, but a mechanistic model incorporating the recently identified islet β cell heterogeneity remains elusive. Here, w...
-
Article
Open AccessCopper assisted sequence-specific chemical protein conjugation at a single backbone amide
Direct, site-specific methods of protein functionalization are highly desirable for biotechnology. However, such methods are challenging due to the difficulty of chemically differentiating a single site within...
-
Article
Uncovering diffusive states of the yeast membrane protein, Pma1, and how labeling method can change diffusive behavior
We present and analyze video-microscopy-based single-particle-tracking measurements of the budding yeast (Saccharomyces cerevisiae) membrane protein, Pma1, fluorescently labeled either by direct fusion to the swi...
-
Article
Fluorogenic DNA-PAINT for faster, low-background super-resolution imaging
DNA-based points accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution microscopy method that can acquire high-fidelity images at nanometer resolution. It suffers, however...
-
Article
Implementation of a 4Pi-SMS super-resolution microscope
The development of single-molecule switching (SMS) fluorescence microscopy (also called single-molecule localization microscopy) over the last decade has enabled researchers to image cell biological structures...
-
Article
Open AccessSpatial distribution of IL4 controls iNKT cell-DC crosstalk in tumors
The spatiotemporal distribution of cytokines orchestrates immune responses in vivo, yet the underlying mechanisms remain to be explored. We showed here that the spatial distribution of interleukin-4 (IL4) in i...
-
Article
Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging
Combining the molecular specificity of fluorescent probes with three-dimensional imaging at nanoscale resolution is critical for investigating the spatial organization and interactions of cellular organelles a...
-
Article
Long time-lapse nanoscopy with spontaneously blinking membrane probes
Super-resolution imaging of the dynamics of organelle structures in live cells is facilitated by blinking, far-red dyes.
-
Article
Open AccessThree-dimensional super-resolution protein localization correlated with vitrified cellular context
We demonstrate the use of cryogenic super-resolution correlative light and electron microscopy (csCLEM) to precisely determine the spatial relationship between proteins and their native cellular structures. Se...
-
Article
Open AccessDrift correction for single-molecule imaging by molecular constraint field, a distance minimum metric
The recent developments of far-field optical microscopy (single molecule imaging techniques) have overcome the diffraction barrier of light and improve image resolution by a factor of ten compared with convent...
-
Article
Super-resolution microscopy of live cells using single molecule localization
The resolution of conventional light microscopy is insufficient for subcelluar studies. The invention of various super-resolution imaging techniques breaks the diffraction barrier and pushes the resolution lim...
-
Article
Ultrafast, accurate, and robust localization of anisotropic dipoles
The resolution of single molecule localization imaging techniques largely depends on the precision of localization algorithms. However, the commonly used Gaussian function is not appropriate for anisotropic di...
-
Article
C13C4.5/Spinster, an evolutionarily conserved protein that regulates fertility in C. elegans through a lysosome-mediated lipid metabolism process
Lipid droplets, which are conserved across almost all species, are cytoplasmic organelles used to store neutral lipids. Identification of lipid droplet regulators will be conducive to resolving obesity and oth...
-
Article
Rational design of true monomeric and bright photoactivatable fluorescent proteins
Structure determination followed by targeted engineering of the popular photoactivatable fluorescent protein monomeric (m)Eos2 yields mEos3 versions that are more monomeric and less disruptive in protein fusio...
-
Article
Open AccessRAB-27 and its effector RBF-1 regulate the tethering and docking steps of DCV exocytosis in C. elegans
The molecular mechanisms by which dense core vesicles (DCVs) translocate, tether, dock and prime are poorly understood. In this study, Caenorhabditis elegans was used as a model organism to study the function of ...
