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Article
Hydrolysis of protein in vacuoles isolated from higher plant tissue
THE vacuoles of higher plant cells are known to be sites of deposition of salts and metabolites. A role as the lytic compartment has also been proposed1, and Matile has argued that the vacuole participates in the...
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Article
Molecular structure and subcellular localization of spinach leaf glycolate oxidase
Glycolate oxidase (E.C. 1.1.3.1) was purified from spinach leaves (Spinacia oleracea). The molecular weight of the native protein was determined by sucrose density gradient centrifugation to be 290,000 daltons (1...
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Article
Molecular structure and subcellular localization of spinach leaf glycolate oxidase
Glycolate oxidase (E.C. 1.1.3.1) was purified from spinach leaves (Spinacia oleracea). The molecular weight of the native protein was determined by sucrose density gradient centrifugation to be 290,000 daltons (1...
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Article
The presequence of the precursor to the nucleus-encoded 30 kDa protein of photosystem II in Euglena gracilis Z includes two hydrophobic domains
A cDNA clone for the extrinsic 30 kDa protein (OEC30) of photosystem II in Euglena gracilis Z was isolated and characterized. The open reading frame of the cDNA encoded a polypeptide of 338 amino acids, which con...
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Chapter
Molecular Characterization of Aconitase in Etiolated Pumpkin Cotyledons
Aconitase (EC 4.2.1.3) is an enzyme that catalyzes the reaction of reversible conversion between citrate and isocitrate. In cotyledons of oil-storage plants such as pumpkin, aconitase was thought to exist not ...
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Article
Molecular characterization of a glyoxysomal citrate synthase that is synthesized as a precursor of higher molecular mass in pumpkin
A cDNA clone for glyoxysomal citrate synthase (gCS) was isolated from a λgt11 cDNA library prepared from etiolated pumpkin cotyledons. The cDNA of 1989 bp consisted of a 1548 bp open reading frame that encoded...
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Article
Characterization of two integral membrane proteins located in the protein bodies of pumpkin seeds
Two integral membrane proteins, MP28 and MP23, were found in protein bodies isolated from pumpkin (Cucurbita sp.) seeds. Molecular characterization revealed that both MP28 and MP23 belong to the seed TIP (tonopla...
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Article
Homologues of a vacuolar processing enzyme that are expressed in different organs in Arabidopsis thaliana
Vacuolar processing enzymes (VPEs) are responsible for the maturation of seed proteins. These processing enzymes belong to a novel group of cysteine proteinases with molecular masses of 37 to 39 kDa. We isolat...
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Article
Pumpkin hydroxypyruvate reductases with and without a putative C-terminal signal for targeting to microbodies may be produced by alternative splicing
Two full-length cDNAs encoding hydroxypyruvate reductase were isolated from a cDNA library constructed with poly(A)+ RNA from pumpkin green cotyledons. One of the cDNAs, designated HPR1, encodes a polypeptide of ...
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Article
cDNA cloning and expression of a gene for 3-ketoacyl-CoA thiolase in pumpkin cotyledons
A cDNA clone for 3-ketoacyl-CoA thiolase (EC 2.3.1.16) was isolated from a λgt11 cDNA library constructed from the poly(A)+ RNA of etiolated pumpkin cotyledons. The cDNA insert contained 1682 nucleotides and enco...
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Article
cDNA cloning and differential gene expression of three catalases in pumpkin
Three cDNA clones (cat1, cat2, cat3) for catalase (EC 1.11.1.6) were isolated from a cDNA library of pumpkin (Cucurbita sp.) cotyledons. In northern blotting using the cDNA-specific probe, the cat1 mRNA levels...
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Article
Microbody defective mutants of arabidopsis
In germinating fatty seedlings, microbodies are differentiated to leaf peroxisomes from glyoxysomes during greening, and then transformed to glyoxysomes from leaf peroxisomes during senescence. These transform...
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Article
Microbody proliferation and segregation cycle in the single-microbody alga Cyanidioschyzon merolae
The proliferation cycle of the microbody was studied in the primitive red alga Cyanidioschyzon merolae, which contains one microbody per cell. Cells were synchronized with a dark/light cycle, and the morphology ...
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Article
A leaf-peroxisomal protein, hydroxypyruvate reductase, is produced by light-regulated alternative splicing
Hydroxypyruvate reductase (HPR) is localized in leaf peroxisomes in plants, and it plays an important role in the glycolate pathway of photorespiration. In this laboratory, two highly homologous cDNAs for pump...
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Article
Transport of peroxisomal proteins synthesized as large precursors in plants
Plant peroxisomes contain at least four proteins, namely, citrate synthase, malate dehydrogenase, long-chain acyl-CoA oxidase, and 3-ketoacyl-CoA thiolase, which are synthesized as large precursors with an N-t...
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Article
Functional transformation of plant peroxisomes
Peroxisomes in higher plant cells are known to differentiate into at least three different classes, namely, glyoxysomes, leaf peroxisomes, and unspecialalized peroxisomes, dependending on the cell types. In ge...
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Chapter
Genetic Approaches to Understand Plant Peroxisomes
Peroxisomes in higher plant cells are known to differentiate in function depending on the cell type. Because of the functional differentiation, plant peroxisomes are sub-divided into at least three different c...
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Article
The slow wound-response of γVPE is regulated by endogenous salicylic acid in Arabidopsis
Vacuolar processing enzyme (VPE) is a cysteine protease responsible for the maturation of various vacuolar proteins in higher plants. The Arabidopsis thaliana (L.) Heynh. γVPE gene, encoding a VPE homologue, is s...
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Article
Identification of the OsOPR7 gene encoding 12-oxophytodienoate reductase involved in the biosynthesis of jasmonic acid in rice
Enzyme 12-oxophytodienoate (OPDA) reductase (EC1.3.1.42), which is involved in the biosynthesis of jasmonic acid (JA), catalyses the reduction of 10, 11-double bonds of OPDA to yield 3-oxo-2-(2′-pentenyl)-cycl...
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Article
A possible involvement of autophagy in amyloplast degradation in columella cells during hydrotropic response of Arabidopsis roots
Seedling roots display not only gravitropism but also hydrotropism, and the two tropisms interfere with one another. In Arabidopsis (Arabidopsis thaliana) roots, amyloplasts in columella cells are rapidly degrade...
